##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	he_in180bp_2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	165090082	
Filtered Sequences	0	
Sequence length	100	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.752682998849078	31.0	31.0	34.0	27.0	34.0
2	30.93273608041457	33.0	31.0	34.0	27.0	34.0
3	30.997499674147598	33.0	31.0	34.0	27.0	34.0
4	34.401296136008945	37.0	35.0	37.0	30.0	37.0
5	34.376300418822254	37.0	35.0	37.0	30.0	37.0
6	34.39033052270214	37.0	35.0	37.0	30.0	37.0
7	34.38699819653612	37.0	35.0	37.0	30.0	37.0
8	34.367301337944696	37.0	35.0	37.0	30.0	37.0
9	35.82387848714013	39.0	35.0	39.0	30.0	39.0
10-14	36.03234244683457	39.2	35.4	39.4	30.0	39.4
15-19	36.8658365206942	40.0	37.0	41.0	30.0	41.0
20-24	36.719915731824514	40.0	36.6	41.0	29.8	41.0
25-29	36.435727441216	40.0	36.0	41.0	29.0	41.0
30-34	36.216459229816124	39.0	36.0	41.0	28.2	41.0
35-39	36.13266684185184	39.8	36.0	41.0	27.4	41.0
40-44	35.90678053209762	39.0	35.4	41.0	27.0	41.0
45-49	35.50630529700748	39.0	35.0	41.0	26.0	41.0
50-54	34.62804475558986	38.0	34.0	40.0	24.2	40.6
55-59	34.704361473392446	38.0	34.0	40.0	24.0	41.0
60-64	34.41745213501075	37.8	34.0	40.0	24.2	41.0
65-69	33.623645252050935	36.6	33.2	39.4	22.6	41.0
70-74	32.73375390897195	35.4	32.4	38.4	21.6	40.4
75-79	31.851268856962587	35.0	32.0	36.8	19.8	39.2
80-84	31.05247809495909	35.0	31.0	36.0	17.6	37.6
85-89	30.33879566793116	34.8	31.0	35.0	9.8	36.4
90-94	29.72077542005219	34.0	30.8	35.0	3.0	36.0
95-99	28.961739820324276	34.0	30.0	35.0	2.0	35.2
100	27.435748441871876	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1981734.0
3	423728.0
4	367344.0
5	362677.0
6	379505.0
7	411490.0
8	467062.0
9	542163.0
10	617874.0
11	655177.0
12	655624.0
13	639165.0
14	618347.0
15	613114.0
16	616083.0
17	633398.0
18	661573.0
19	706465.0
20	772756.0
21	863311.0
22	978932.0
23	1127766.0
24	1301171.0
25	1512461.0
26	1756895.0
27	2055444.0
28	2419188.0
29	2869365.0
30	3447050.0
31	4200026.0
32	5214826.0
33	6636318.0
34	8784923.0
35	1.2374716E7
36	1.9373994E7
37	3.2172619E7
38	3.5156422E7
39	1.0661082E7
40	58294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.557811930185917	33.30333116007992	32.26628769797952	16.872569211754634
2	17.887924899031407	35.23569821683952	33.53348686325309	13.342890020875986
3	17.19779705264369	34.95541030216084	32.08562753876228	15.76116510643318
4	18.85318489819522	33.26720164381952	30.624533928850127	17.25507952913513
5	19.601848949579477	31.791860776861547	29.994283594716105	18.612006678842867
6	19.073302392074222	31.59354329345989	29.337499842017206	19.995654472448685
7	19.808070194139614	31.3126596790899	29.812827452805067	19.066442673965426
8	18.82699019444301	30.61043994902914	30.974335539772362	19.588234316755486
9	18.822480134763072	30.1697406741419	31.67994844714203	19.327830743952997
10-14	18.436299389711458	31.22621075678304	31.72111205201255	18.616377801492952
15-19	18.79315922793782	30.864015294272917	31.314385831476976	19.028439646312282
20-24	18.706742480806792	31.085692300473987	31.412320236464346	18.795244982254868
25-29	19.12393724047018	30.80978877651862	30.898473605953274	19.167800377057922
30-34	18.939176353617206	30.84328787986197	31.2845936958849	18.932942070635928
35-39	19.202740459863612	30.713908682421525	30.733351368901623	19.34999948881324
40-44	18.9103380592557	30.921955846445165	31.131376690459135	19.036329403839996
45-49	19.20541621884178	30.989708868507677	30.65498097162977	19.149893941020775
50-54	18.884006868677748	31.00457571329056	31.102111340843898	19.009306077187794
55-59	19.053225582731727	31.13646952022357	30.8091849775279	19.001119919516803
60-64	18.80110074525316	31.257757333361536	31.202407046135722	18.738734875249584
65-69	18.943033074771144	31.127410536506943	31.03601013477108	18.89354625395083
70-74	18.76694855953845	31.258252931931697	31.203957701730268	18.770840806799587
75-79	18.766606393132765	31.27596239453057	31.150075011487743	18.807356200848915
80-84	18.636021536782913	31.20494642024016	31.484604202747825	18.674427840229107
85-89	18.76936602682923	31.301357462103006	31.15341038563391	18.77586612543385
90-94	18.713048863968254	31.141004065455068	31.35555931588257	18.79038775469411
95-99	18.72625370013261	31.145335500009036	31.328316628352624	18.800094171505723
100	18.868056138154678	31.18405988189493	31.162140039522786	18.785743940427608
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	34.43038114194055
2	31.23081491990739
3	32.95896215907687
4	36.108264427330354
5	38.21385562842235
6	39.06895686452291
7	38.874512868105036
8	38.415224511198495
9	38.15031087871607
10-14	37.05267719120441
15-19	37.82159887425011
20-24	37.50198746306167
25-29	38.291737617528106
30-34	37.87211842425313
35-39	38.55273994867685
40-44	37.94666746309569
45-49	38.355310159862555
50-54	37.893312945865546
55-59	38.05434550224853
60-64	37.53983562050274
65-69	37.83657932872197
70-74	37.53778936633804
75-79	37.57396259398168
80-84	37.31044937701202
85-89	37.545232152263075
90-94	37.50343661866237
95-99	37.526347871638336
100	37.653800078582286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137158.0
1	77380.0
2	14141.0
3	10105.0
4	9483.5
5	9857.5
6	11007.5
7	12528.5
8	14663.0
9	17700.0
10	21782.0
11	27318.5
12	35005.5
13	45606.0
14	58983.5
15	77239.0
16	105103.5
17	148564.0
18	209268.0
19	297675.5
20	425474.0
21	594501.5
22	813848.5
23	1096714.0
24	1448351.5
25	1877265.0
26	2351847.0
27	2871227.5
28	3483857.5
29	4216577.0
30	5063049.5
31	6003752.5
32	7082301.0
33	8297049.5
34	9390376.0
35	9956202.0
36	9919781.5
37	9404370.5
38	8551631.0
39	7550133.0
40	6555137.5
41	5669357.0
42	4901313.0
43	4275147.0
44	3888233.0
45	3820099.5
46	4048708.0
47	4434320.5
48	4736429.0
49	4730788.5
50	4321450.0
51	3590419.5
52	2737627.0
53	1957068.0
54	1340958.5
55	895576.0
56	585471.0
57	373219.5
58	230168.0
59	134310.5
60	73171.0
61	38985.5
62	22317.5
63	14202.5
64	9778.5
65	7023.0
66	5205.0
67	3931.5
68	3082.5
69	2456.0
70	1981.5
71	1609.5
72	1341.0
73	1147.0
74	963.5
75	822.0
76	713.0
77	627.5
78	560.0
79	502.5
80	448.5
81	423.5
82	390.0
83	342.5
84	315.5
85	273.5
86	243.0
87	227.0
88	196.5
89	160.0
90	144.5
91	128.5
92	91.0
93	57.0
94	40.5
95	34.5
96	21.5
97	12.5
98	6.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19303703538047792
2	0.23119923097500186
3	0.2609841819570966
4	0.2590846129690577
5	0.2661607497414654
6	0.12036458980013107
7	0.1472190194926428
8	0.14573800987027194
9	0.12390689829568322
10-14	0.13286540132677382
15-19	0.1379509884791262
20-24	0.10680544698015233
25-29	0.11888382246972291
30-34	0.13002961619463005
35-39	0.13255381386266438
40-44	0.13375727804169363
45-49	0.10317712483782035
50-54	0.08883743845981008
55-59	0.12332079403776659
60-64	0.1107867885122257
65-69	0.11411939331400901
70-74	0.11071446436134182
75-79	0.10507935903744962
80-84	0.11134430231853662
85-89	0.11944921076482352
90-94	0.11265619215090099
95-99	0.11000091453101345
100	0.10911436823927435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.65090082E8
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	48.67257454928523
#Duplication Level	Relative count
1	100.0
2	19.24925735889819
3	7.09424790710235
4	4.141686920514898
5	2.9309568818075435
6	2.2441263840129624
7	1.7580340264650283
8	1.4267710865064362
9	1.2179314069673237
10++	39.971194526960126
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGGCC	27297455	4.4083962	6.753119	4
GGCCT	27248630	4.405466	6.296529	6
CGCGC	14592925	3.9105976	4.933435	6
GCGCG	14542255	3.8902445	4.9358234	25-29
AGCGC	21761125	3.514308	5.181614	4
GCGCT	20957960	3.3884118	4.197989	7
GCGCC	12215960	3.2736208	4.3651156	15-19
CACGT	32360215	3.1584349	3.9180355	5
GGCGC	11700105	3.1299322	3.9992266	50-54
ACGTG	31194115	3.0393276	3.7371948	6
AGTCC	30944705	3.0202777	3.8059008	3
>>END_MODULE