##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	he_in180bp_1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	165090082	
Filtered Sequences	0	
Sequence length	100	
%GC	37	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.679316247477544	31.0	31.0	34.0	27.0	34.0
2	30.997017828121255	31.0	31.0	34.0	27.0	34.0
3	31.118997548259742	31.0	31.0	34.0	27.0	34.0
4	34.63460571786499	37.0	35.0	37.0	32.0	37.0
5	34.24280661511816	37.0	35.0	37.0	30.0	37.0
6	34.16176422397076	37.0	35.0	37.0	30.0	37.0
7	34.0889988230789	37.0	35.0	37.0	30.0	37.0
8	34.03418738383085	37.0	35.0	37.0	30.0	37.0
9	35.48777027077859	39.0	35.0	39.0	30.0	39.0
10-14	35.640445065621805	39.2	35.2	39.4	30.0	39.4
15-19	36.4477839571247	40.0	36.0	41.0	29.2	41.0
20-24	36.318322842676885	40.0	36.0	41.0	28.8	41.0
25-29	36.007001321860145	39.6	36.0	41.0	27.2	41.0
30-34	35.666761722245674	39.0	35.4	41.0	26.8	41.0
35-39	35.25624717904011	39.0	35.0	40.4	25.2	41.0
40-44	34.82105188729629	38.2	34.0	40.0	24.2	41.0
45-49	34.40895482625056	38.0	33.2	40.0	23.2	41.0
50-54	34.54191911177317	38.4	33.8	40.0	23.0	41.0
55-59	34.20925287807417	38.0	33.6	40.0	22.4	41.0
60-64	33.528135461220494	37.4	32.8	40.0	20.8	41.0
65-69	32.655161185273386	36.2	32.0	39.2	18.2	41.0
70-74	31.69649122592356	35.0	31.0	37.8	11.8	40.0
75-79	30.501377784766014	34.4	30.0	36.2	7.2	39.0
80-84	30.1613287223396	34.4	30.0	35.6	2.6	37.6
85-89	29.425434688438763	34.0	29.6	35.0	2.0	36.4
90-94	28.699555115612576	34.0	29.0	35.0	2.0	36.0
95-99	27.80463977478671	34.0	28.6	35.0	2.0	35.0
100	26.23347504909471	32.0	25.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3626269.0
3	1142120.0
4	591245.0
5	448890.0
6	388758.0
7	393712.0
8	435044.0
9	487311.0
10	535459.0
11	568154.0
12	590499.0
13	613782.0
14	644442.0
15	680367.0
16	711515.0
17	735769.0
18	752418.0
19	766076.0
20	784992.0
21	824534.0
22	901448.0
23	1034522.0
24	1228806.0
25	1488163.0
26	1806429.0
27	2186856.0
28	2641167.0
29	3183098.0
30	3853574.0
31	4707128.0
32	5827171.0
33	7378227.0
34	9658957.0
35	1.3307244E7
36	1.983508E7
37	3.0557758E7
38	3.1203476E7
39	8532372.0
40	37250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.77678696239892	32.85918486492515	32.34011902417866	17.023909148497264
2	17.819067649071584	34.70282405510011	34.08115010785829	13.396958187970007
3	17.107668430770644	34.61560949131345	32.57625563715167	15.700466440764243
4	18.85863310928645	33.267681928250234	30.939752684319977	16.933932278143335
5	19.492984357830142	31.810702710780603	30.16018136066922	18.536131570720038
6	19.001147879913617	31.655004328154735	29.452992257982586	19.89085553394906
7	19.852295642576383	31.346929492322428	29.79930569205356	19.00146917304762
8	18.880497695794908	30.527110134913237	30.95004798119925	19.642344188092604
9	18.8731749408421	30.100069391973562	31.66611939329456	19.360636273889774
10-14	18.5369412068597	31.19851089742596	31.613885564337608	18.650662331376726
15-19	18.872821786682383	30.85418165427895	31.183574233069557	19.08942232596911
20-24	18.75860083132663	31.08690361649501	31.335493909134627	18.819001643043734
25-29	19.209831048210745	30.787228218954365	30.81628043491287	19.186660297922018
30-34	18.982317685957895	30.82100649076562	31.260909035973842	18.935766787302647
35-39	19.28746202719622	30.696980464828172	30.65057783773827	19.364979670237343
40-44	18.985458946068068	30.900268776071293	31.09376676833191	19.02050550952873
45-49	19.277314335100915	30.979068794473484	30.595183929619342	19.148432940806256
50-54	18.93450519735244	30.957911012513943	31.095610896418673	19.01197289371494
55-59	19.126019439716046	31.069094220259835	30.794229332054567	19.01065700796955
60-64	18.86885827563665	31.212300696712376	31.20275933259596	18.716081695055017
65-69	19.047867268585634	31.10050794372658	30.97645687143611	18.875167916251677
70-74	18.864082080831853	31.22396797240524	31.157148601307505	18.754801345455398
75-79	18.82872853327878	31.2436513546898	31.10272269751241	18.824897414519015
80-84	18.712161040448073	31.146827712388465	31.468182822163232	18.67282842500023
85-89	18.827935198973407	31.276993327141966	31.080635290118813	18.81443618376581
90-94	18.78161760845367	31.088338331426264	31.302251017201577	18.827793042918493
95-99	18.795859697700077	31.13193395133483	31.211974824938814	18.860231526026283
100	18.878436642487415	31.1114890223548	31.132322803190693	18.877751531967093
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	34.80069611089618
2	31.216025837041595
3	32.80813487153489
4	35.79256538742979
5	38.029115928550176
6	38.89200341386268
7	38.853764815624004
8	38.52284188388751
9	38.23381121473188
10-14	37.18760353823643
15-19	37.9622441126515
20-24	37.577602474370366
25-29	38.39649134613276
30-34	37.91808447326054
35-39	38.65244169743357
40-44	38.005964455596796
45-49	38.42574727590717
50-54	37.946478091067384
55-59	38.136676447685595
60-64	37.584939970691664
65-69	37.9230351848373
70-74	37.61888342628725
75-79	37.6536259477978
80-84	37.38498946544831
85-89	37.64237138273922
90-94	37.60941065137216
95-99	37.656091223726364
100	37.75618817445451
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9322.0
1	5587.5
2	1978.0
3	2494.0
4	3453.0
5	4528.0
6	5671.0
7	7137.5
8	9153.5
9	11653.5
10	15033.5
11	19842.5
12	26562.0
13	35909.5
14	48565.0
15	65982.0
16	92170.0
17	133905.0
18	193295.0
19	279456.0
20	404925.0
21	572193.0
22	789877.0
23	1072155.0
24	1424751.5
25	1856962.0
26	2333937.5
27	2854828.5
28	3469592.0
29	4206273.0
30	5057591.0
31	5996918.5
32	7074261.5
33	8293810.0
34	9397178.5
35	9967018.5
36	9926391.0
37	9414032.0
38	8565814.0
39	7565718.5
40	6567917.5
41	5684572.0
42	4920945.5
43	4295770.5
44	3914724.0
45	3852729.5
46	4086343.0
47	4470555.5
48	4767774.0
49	4754487.5
50	4334685.5
51	3596694.0
52	2738964.5
53	1959331.0
54	1349799.5
55	908886.5
56	601230.5
57	389061.5
58	244704.0
59	148244.5
60	85571.0
61	48862.0
62	29762.0
63	20020.5
64	14683.0
65	11366.5
66	9125.0
67	7625.0
68	6526.0
69	5691.5
70	5053.5
71	4425.0
72	4051.5
73	3805.0
74	3481.0
75	3247.0
76	3094.5
77	2900.0
78	2709.0
79	2523.5
80	2306.5
81	2137.5
82	1996.5
83	1787.5
84	1604.5
85	1451.5
86	1241.5
87	1049.0
88	857.5
89	707.0
90	573.5
91	420.5
92	282.0
93	188.5
94	124.5
95	75.5
96	40.0
97	17.5
98	8.5
99	3.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04647099272747347
2	0.011987394857554193
3	0.004602335832627426
4	0.008880606165063266
5	0.045582386954050945
6	0.01825245928462256
7	0.04201221488278139
8	0.02297775828835072
9	0.015944628339332946
10-14	0.016883630841009575
15-19	0.02517098513525482
20-24	0.007972253596675784
25-29	0.009438967993243834
30-34	0.013944871624692753
35-39	0.01636306655901958
40-44	0.023851947689989033
45-49	0.025473244358798008
50-54	0.024304549076424833
55-59	0.032939107753305254
60-64	0.025684886388268918
65-69	0.02500877066618696
70-74	0.015550903899847843
75-79	0.018927121254927962
80-84	0.027484873379613438
85-89	0.027530303122630953
90-94	0.015299041404558754
95-99	0.01948233328759265
100	0.004378215767074366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.65090082E8
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	49.81527547447945
#Duplication Level	Relative count
1	100.0
2	19.89150090415913
3	7.280087386750626
4	4.396875372908271
5	3.0383418547654233
6	2.3957922178059685
7	1.8496250263904315
8	1.5191709273827116
9	1.2740841372853196
10++	41.940132732396435
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGGCC	27922905	4.474077	6.6418395	4
GGCCT	27835010	4.464202	6.401457	6
CGCGC	14717660	3.905474	4.912562	25-29
GCGCG	14756375	3.8979793	5.008568	25-29
AGCGC	21755585	3.4858897	5.025947	4
GCGCT	20985910	3.3657382	4.1661787	7
GCGCC	12195890	3.2362978	4.2501516	15-19
CACGT	32552835	3.1668267	3.9193087	5
GGCGC	11873690	3.1365016	3.9907246	50-54
ACGTG	31134620	3.0151155	3.8033173	6
>>END_MODULE