FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | EL589_6KB_2.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 19974112 |
| Filtered Sequences | 0 |
| Sequence length | 100 |
| %GC | 39 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
![[FAIL]](Icons/error.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG | 48276 | 0.24169284722144344 | Illumina Single End PCR Primer 1 (98% over 50bp) |
| AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA | 36104 | 0.18075396793609647 | No Hit |
| ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC | 33643 | 0.1684330197007006 | No Hit |
| GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 31616 | 0.15828488395378978 | Illumina Single End PCR Primer 1 (100% over 50bp) |
| CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC | 30055 | 0.15046976806778695 | No Hit |
| GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG | 28074 | 0.140551930418734 | No Hit |
| CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT | 27235 | 0.13635149337302205 | No Hit |
| TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA | 24253 | 0.12142216885536639 | No Hit |
| GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG | 23543 | 0.11786756777973409 | No Hit |
| AGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG | 22120 | 0.11074334618730484 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 5708520 | 8.207205 | 54.943783 | 40-44 |
| AAAAA | 26864190 | 5.067826 | 22.625902 | 60-64 |
| GCCGG | 3058180 | 4.9273143 | 14.50433 | 45-49 |
| CCGGC | 2698335 | 4.602362 | 5.494613 | 45-49 |
| TCCCG | 3378720 | 4.0293775 | 4.472445 | 85-89 |
| CGCCG | 2324255 | 3.9643202 | 19.899734 | 45-49 |
| CGGGA | 3594075 | 3.6441126 | 21.731857 | 4 |
| AACCC | 4695615 | 3.5544133 | 4.4480076 | 1 |
| TTTTT | 14609990 | 3.5101497 | 3.9918294 | 3 |
| CCGGG | 2166515 | 3.4906707 | 7.134299 | 35-39 |
| CCCGG | 2038860 | 3.4775414 | 5.136942 | 45-49 |
| GCCCG | 1997380 | 3.4067922 | 8.489621 | 45-49 |
| CCCGT | 2777900 | 3.3128543 | 4.803688 | 45-49 |
| CGGGC | 1941390 | 3.1279511 | 4.3606486 | 4 |
| ACCCT | 3856050 | 3.0635405 | 3.8902242 | 2 |
| GGGCC | 1815755 | 2.9255292 | 9.705959 | 45-49 |
| ACGGG | 2883870 | 2.9240205 | 8.245729 | 3 |
| CGGCG | 1764270 | 2.842577 | 8.000075 | 10-14 |
| GGGAA | 4411195 | 2.8146114 | 15.1244335 | 20-24 |
| GGCCC | 1593675 | 2.7182205 | 7.0905423 | 45-49 |
| TAGGG | 3854920 | 2.5815656 | 8.356589 | 15-19 |
| GCGTC | 2266605 | 2.5534294 | 9.52399 | 10-14 |
| GCGGG | 1638510 | 2.4937823 | 10.10947 | 10-14 |
| AGGGT | 3690405 | 2.4713929 | 6.270536 | 25-29 |
| CCGTA | 3230290 | 2.424291 | 6.0873523 | 45-49 |
| GAAAA | 8425850 | 2.3859777 | 5.2810225 | 6 |
| CGGGG | 1561940 | 2.377244 | 13.724987 | 35-39 |
| TCGCC | 1978800 | 2.3598676 | 7.866067 | 45-49 |
| GGAAA | 5440485 | 2.3125708 | 8.3415365 | 6 |
| GGCCG | 1383490 | 2.2290676 | 6.197025 | 45-49 |
| GCGCC | 1259220 | 2.1477637 | 13.917253 | 45-49 |
| GGCGC | 1251500 | 2.0164063 | 12.641491 | 40-44 |
| TAAAA | 10013960 | 1.9827106 | 6.026614 | 55-59 |
| TCATT | 5699150 | 1.9752277 | 5.3462186 | 50-54 |
| GGGGC | 1288840 | 1.9615911 | 14.959638 | 40-44 |
| TTAAA | 9283130 | 1.9290942 | 6.017352 | 55-59 |
| GGGGA | 2003080 | 1.9185152 | 13.775548 | 20-24 |
| TCGGG | 1802155 | 1.917796 | 15.208315 | 3 |
| AGGGG | 1988075 | 1.9041436 | 11.132436 | 25-29 |
| GGGAG | 1984575 | 1.9007916 | 15.922971 | 5 |
| GGGCG | 1233265 | 1.877007 | 12.510993 | 40-44 |
| CTCGG | 1564240 | 1.7621845 | 12.316735 | 35-39 |
| GGCGG | 1082065 | 1.6468831 | 10.12525 | 10-14 |
| GAAAG | 3812765 | 1.6206807 | 6.534251 | 7 |
| GGAAG | 2514460 | 1.6043788 | 34.545906 | 5 |
| GGGTA | 2326700 | 1.558146 | 5.3635235 | 25-29 |
| GCCGT | 1381020 | 1.5557792 | 7.189211 | 45-49 |
| AGCGT | 2160660 | 1.5317643 | 5.953701 | 10-14 |
| GAGGG | 1570450 | 1.5041497 | 17.33256 | 9 |
| AAAGG | 3394980 | 1.4430943 | 6.338612 | 8 |
| CCGGA | 1335650 | 1.4336233 | 5.791753 | 45-49 |
| AGGGA | 2126830 | 1.3570471 | 5.841641 | 20-24 |
| AAGAG | 3096380 | 1.3161693 | 23.015337 | 7 |
| CGTCG | 1143150 | 1.2878083 | 8.530725 | 10-14 |
| CGGAA | 1846140 | 1.2469947 | 38.79205 | 4 |
| GAACG | 1801420 | 1.2167879 | 5.96886 | 1 |
| GAAGA | 2788960 | 1.1854949 | 23.880285 | 6 |
| CGTGT | 1568615 | 1.1671519 | 5.389876 | 15-19 |
| GAGCG | 1139280 | 1.1551416 | 50.335487 | 9 |
| AGAGG | 1793245 | 1.1441997 | 11.816692 | 8 |
| GCGGC | 683730 | 1.10162 | 6.504952 | 10-14 |
| CGCCC | 597110 | 1.0781447 | 5.406834 | 45-49 |
| GGTGG | 1054185 | 1.0597156 | 6.5631056 | 40-44 |
| GATCG | 1487605 | 1.0546129 | 44.767246 | 1 |
| ATCGG | 1482065 | 1.0506856 | 44.55644 | 2 |
| TCGGA | 1465005 | 1.0385911 | 36.959904 | 3 |
| AACGG | 1460020 | 0.9861859 | 5.513825 | 2 |
| CGGTG | 905035 | 0.96310943 | 5.726893 | 35-39 |
| AGAGC | 1416140 | 0.95654655 | 34.016804 | 8 |
| AAGGG | 1483970 | 0.94686335 | 8.369261 | 9 |
| GTCGC | 839450 | 0.94567704 | 6.0647 | 40-44 |
| AAGCG | 1378270 | 0.930967 | 5.39337 | 9 |
| GGAGA | 1361160 | 0.86850303 | 11.72249 | 6 |
| GTAGG | 1294555 | 0.86693853 | 6.342189 | 15-19 |
| TCTCG | 1089975 | 0.85854954 | 7.246272 | 35-39 |
| GAGAG | 1333610 | 0.85092455 | 10.753165 | 7 |
| GGTCG | 742705 | 0.790363 | 5.670244 | 40-44 |
| ATCTC | 1367700 | 0.7176867 | 5.1502743 | 35-39 |