##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL589_6KB_2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	19974112	
Filtered Sequences	0	
Sequence length	100	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02820020234191	33.0	31.0	34.0	28.0	34.0
2	31.08954290433537	34.0	31.0	34.0	28.0	34.0
3	31.060590177926308	34.0	31.0	34.0	27.0	34.0
4	34.37386913620991	37.0	35.0	37.0	32.0	37.0
5	34.39865471866784	37.0	35.0	37.0	32.0	37.0
6	34.52677395620891	37.0	35.0	37.0	32.0	37.0
7	34.18698107830776	37.0	35.0	37.0	30.0	37.0
8	34.35924750997692	37.0	35.0	37.0	32.0	37.0
9	35.64055107931707	39.0	37.0	39.0	30.0	39.0
10-14	35.89009046309543	39.4	37.2	39.4	30.2	39.4
15-19	36.87978872853021	41.0	38.0	41.0	30.4	41.0
20-24	36.79771438149541	40.2	38.0	41.0	29.6	41.0
25-29	36.539498146400696	40.0	38.0	41.0	28.8	41.0
30-34	36.13126376782107	40.0	38.0	41.0	25.8	41.0
35-39	36.11643652543853	40.0	38.0	41.0	26.4	41.0
40-44	35.85471324081892	40.0	37.2	41.0	25.4	41.0
45-49	35.546056255216754	40.0	36.4	41.0	24.0	41.0
50-54	34.72917749735257	39.0	35.0	40.6	22.2	41.0
55-59	34.55139846016684	38.8	35.0	41.0	21.6	41.0
60-64	34.03785209575274	37.4	34.0	40.2	21.8	41.0
65-69	33.13834090847193	36.4	34.0	39.2	17.0	41.0
70-74	32.352667372647154	35.2	34.0	37.8	13.0	40.2
75-79	31.59267669070845	35.0	33.2	36.4	8.6	38.6
80-84	30.98397377565521	35.0	33.0	35.6	4.6	37.0
85-89	30.543898011586197	35.0	33.0	35.0	2.0	36.0
90-94	30.14377295971906	35.0	32.2	35.0	2.0	36.0
95-99	29.685664263823092	35.0	32.0	35.0	2.0	35.2
100	28.606145845182002	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	737122.0
3	203159.0
4	64341.0
5	30730.0
6	58535.0
7	88220.0
8	47738.0
9	33386.0
10	33334.0
11	34337.0
12	33745.0
13	39889.0
14	56883.0
15	66292.0
16	65304.0
17	62969.0
18	61642.0
19	60488.0
20	57216.0
21	56879.0
22	59302.0
23	66983.0
24	78920.0
25	92750.0
26	111874.0
27	134989.0
28	164711.0
29	202342.0
30	250110.0
31	316616.0
32	406597.0
33	535240.0
34	734478.0
35	1134279.0
36	2345709.0
37	4768463.0
38	5436058.0
39	1232063.0
40	10419.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	24.792508097061784	31.706569572034056	24.607656318011763	18.8932660128924
2	18.117404179090492	35.767912771259965	28.44712234003114	17.66756070961841
3	18.044734165970088	30.286456028232163	33.609806959271076	18.059002846526674
4	18.224059222257292	28.7071034747377	30.389360988863984	22.679476314141027
5	22.509501298480753	28.953422309837855	29.89171683827546	18.645359553405928
6	23.594900788872774	29.034162792059902	28.3905483459305	18.980388073136822
7	21.106417756491044	32.544042693088706	28.476294570576577	17.873244979843676
8	18.139664982353157	35.1531472337794	28.50325961925116	18.20392816461628
9	22.53960024167577	30.115969525782386	28.85362014007502	18.490810092466816
10-14	21.329499621338762	30.39888557411289	29.269160233007106	19.00245457154124
15-19	21.870244940927087	28.75628699511441	29.929088990040846	19.444379073917656
20-24	22.80765602371655	29.684857004167164	29.07569968384296	18.431787288273327
25-29	20.80750011713638	32.27665952212341	28.420365590284703	18.495474770455502
30-34	22.570986119955016	29.640823148793537	29.292545567840545	18.4956451634109
35-39	18.893044484445877	30.042692192984056	30.84939827933307	20.214865043236998
40-44	23.71447373149868	28.481133489491008	28.854855977378225	18.949536801632085
45-49	21.260040513896975	28.39303686815911	28.78994299989251	21.556979618051407
50-54	20.378806581520248	29.21371970701503	30.021643145767783	20.385830565696942
55-59	18.682928756338217	31.185167436011223	31.19852439057029	18.933379417080268
60-64	18.626237951021388	34.51447031289363	28.087981462345518	18.77131027373946
65-69	18.714602801223112	33.924114722669096	28.743443192190664	18.617839283917125
70-74	19.14986506505947	32.39662199983404	29.056668429377442	19.396844505729057
75-79	19.565319198906042	31.5035328641806	29.475982386270534	19.45516555064283
80-84	19.88475100093337	30.9601528557324	29.404230488145156	19.750865655189077
85-89	19.913458639650994	30.84625401783152	29.53496229864081	19.705325043876677
90-94	20.191514763338578	30.59253590464982	29.314987774900274	19.900961557111327
95-99	20.329953322886453	30.388299189126126	29.312981382735074	19.968766105252342
100	20.532063507257874	30.328218693039926	29.03511565478048	20.104602144921724
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.68577410995418
2	35.7849648887089
3	36.103737012496765
4	40.903535536398316
5	41.15486085188668
6	42.5752888620096
7	38.97966273633472
8	36.34359314696944
9	41.030410334142594
10-14	40.331954192880005
15-19	41.31462401484474
20-24	41.239443311989874
25-29	39.30297488759188
30-34	41.066631283365915
35-39	39.10790952768288
40-44	42.664010533130764
45-49	42.81702013194838
50-54	40.764637147217194
55-59	37.61630817341848
60-64	37.39754822476085
65-69	37.33244208514024
70-74	38.54670957078852
75-79	39.02048474954887
80-84	39.635616656122444
85-89	39.61878368352767
90-94	40.092476320449904
95-99	40.298719428138796
100	40.636665652179595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	427.0
1	388.0
2	327.0
3	378.5
4	577.5
5	685.0
6	630.5
7	661.0
8	758.5
9	1056.0
10	1276.5
11	1291.0
12	1578.5
13	2424.5
14	3194.5
15	4057.0
16	5590.5
17	7862.5
18	10261.0
19	14845.5
20	22934.5
21	34734.5
22	50334.5
23	68781.0
24	94309.0
25	133010.0
26	195514.0
27	274680.5
28	358109.0
29	451306.0
30	546716.5
31	625569.5
32	675599.5
33	710855.5
34	750055.5
35	785689.0
36	822236.0
37	860568.5
38	860435.0
39	835884.0
40	795006.0
41	771768.0
42	809232.5
43	868768.5
44	929769.0
45	968730.0
46	948933.5
47	868604.0
48	746696.0
49	637656.5
50	560994.0
51	468812.0
52	339708.0
53	230208.0
54	173194.0
55	136191.0
56	113403.5
57	93193.0
58	67640.0
59	45138.5
60	32068.0
61	24002.0
62	19310.5
63	15294.0
64	12329.0
65	10799.5
66	9643.5
67	8281.0
68	7409.5
69	7878.5
70	7573.0
71	5826.5
72	4696.0
73	4218.5
74	3197.5
75	2602.5
76	2114.5
77	1916.0
78	1649.5
79	977.5
80	587.0
81	392.0
82	309.5
83	255.0
84	219.5
85	182.5
86	163.0
87	147.5
88	136.0
89	117.0
90	103.0
91	100.0
92	92.0
93	80.5
94	69.5
95	67.0
96	64.0
97	60.5
98	54.5
99	42.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025733309195422552
2	2.653434605753688E-4
3	0.0014819181949114934
4	0.0
5	0.0
6	2.102721763050092E-4
7	0.0024081170667311766
8	0.0
9	0.0012215812147243392
10-14	0.004256509626059972
15-19	0.012458125798032975
20-24	0.0034234312894610786
25-29	0.008343800214998294
30-34	0.004246496665283543
35-39	0.018604081122605098
40-44	0.03476099463145095
45-49	0.038693084328354624
50-54	0.026975917627777395
55-59	0.034973269399911244
60-64	0.03143268646936595
65-69	0.010623751383791178
70-74	0.0035475920030887983
75-79	0.0018774301455804394
80-84	0.002276947280559957
85-89	0.0020146077082175166
90-94	0.0034855116462749382
95-99	0.002133761941457022
100	7.159266955146741E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9974112E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	92.39139183545879
#Duplication Level	Relative count
1	100.0
2	6.627632355835465
3	2.986616807715017
4	1.9361346191694548
5	1.3518500295217477
6	1.0541724070064948
7	0.8770419208817162
8	0.7429639834678213
9	0.6826904152725841
10++	129.75546152332217
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	48276	0.24169284722144344	Illumina Single End PCR Primer 1 (98% over 50bp)
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA	36104	0.18075396793609647	No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	33643	0.1684330197007006	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	31616	0.15828488395378978	Illumina Single End PCR Primer 1 (100% over 50bp)
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC	30055	0.15046976806778695	No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG	28074	0.140551930418734	No Hit
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT	27235	0.13635149337302205	No Hit
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA	24253	0.12142216885536639	No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG	23543	0.11786756777973409	No Hit
AGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG	22120	0.11074334618730484	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	5708520	8.207205	54.943783	40-44
AAAAA	26864190	5.067826	22.625902	60-64
GCCGG	3058180	4.9273143	14.50433	45-49
CCGGC	2698335	4.602362	5.494613	45-49
TCCCG	3378720	4.0293775	4.472445	85-89
CGCCG	2324255	3.9643202	19.899734	45-49
CGGGA	3594075	3.6441126	21.731857	4
AACCC	4695615	3.5544133	4.4480076	1
TTTTT	14609990	3.5101497	3.9918294	3
CCGGG	2166515	3.4906707	7.134299	35-39
CCCGG	2038860	3.4775414	5.136942	45-49
GCCCG	1997380	3.4067922	8.489621	45-49
CCCGT	2777900	3.3128543	4.803688	45-49
CGGGC	1941390	3.1279511	4.3606486	4
ACCCT	3856050	3.0635405	3.8902242	2
GGGCC	1815755	2.9255292	9.705959	45-49
ACGGG	2883870	2.9240205	8.245729	3
CGGCG	1764270	2.842577	8.000075	10-14
GGGAA	4411195	2.8146114	15.1244335	20-24
GGCCC	1593675	2.7182205	7.0905423	45-49
TAGGG	3854920	2.5815656	8.356589	15-19
GCGTC	2266605	2.5534294	9.52399	10-14
GCGGG	1638510	2.4937823	10.10947	10-14
AGGGT	3690405	2.4713929	6.270536	25-29
CCGTA	3230290	2.424291	6.0873523	45-49
GAAAA	8425850	2.3859777	5.2810225	6
CGGGG	1561940	2.377244	13.724987	35-39
TCGCC	1978800	2.3598676	7.866067	45-49
GGAAA	5440485	2.3125708	8.3415365	6
GGCCG	1383490	2.2290676	6.197025	45-49
GCGCC	1259220	2.1477637	13.917253	45-49
GGCGC	1251500	2.0164063	12.641491	40-44
TAAAA	10013960	1.9827106	6.026614	55-59
TCATT	5699150	1.9752277	5.3462186	50-54
GGGGC	1288840	1.9615911	14.959638	40-44
TTAAA	9283130	1.9290942	6.017352	55-59
GGGGA	2003080	1.9185152	13.775548	20-24
TCGGG	1802155	1.917796	15.208315	3
AGGGG	1988075	1.9041436	11.132436	25-29
GGGAG	1984575	1.9007916	15.922971	5
GGGCG	1233265	1.877007	12.510993	40-44
CTCGG	1564240	1.7621845	12.316735	35-39
GGCGG	1082065	1.6468831	10.12525	10-14
GAAAG	3812765	1.6206807	6.534251	7
GGAAG	2514460	1.6043788	34.545906	5
GGGTA	2326700	1.558146	5.3635235	25-29
GCCGT	1381020	1.5557792	7.189211	45-49
AGCGT	2160660	1.5317643	5.953701	10-14
GAGGG	1570450	1.5041497	17.33256	9
AAAGG	3394980	1.4430943	6.338612	8
CCGGA	1335650	1.4336233	5.791753	45-49
AGGGA	2126830	1.3570471	5.841641	20-24
AAGAG	3096380	1.3161693	23.015337	7
CGTCG	1143150	1.2878083	8.530725	10-14
CGGAA	1846140	1.2469947	38.79205	4
GAACG	1801420	1.2167879	5.96886	1
GAAGA	2788960	1.1854949	23.880285	6
CGTGT	1568615	1.1671519	5.389876	15-19
GAGCG	1139280	1.1551416	50.335487	9
AGAGG	1793245	1.1441997	11.816692	8
GCGGC	683730	1.10162	6.504952	10-14
CGCCC	597110	1.0781447	5.406834	45-49
GGTGG	1054185	1.0597156	6.5631056	40-44
GATCG	1487605	1.0546129	44.767246	1
ATCGG	1482065	1.0506856	44.55644	2
TCGGA	1465005	1.0385911	36.959904	3
AACGG	1460020	0.9861859	5.513825	2
CGGTG	905035	0.96310943	5.726893	35-39
AGAGC	1416140	0.95654655	34.016804	8
AAGGG	1483970	0.94686335	8.369261	9
GTCGC	839450	0.94567704	6.0647	40-44
AAGCG	1378270	0.930967	5.39337	9
GGAGA	1361160	0.86850303	11.72249	6
GTAGG	1294555	0.86693853	6.342189	15-19
TCTCG	1089975	0.85854954	7.246272	35-39
GAGAG	1333610	0.85092455	10.753165	7
GGTCG	742705	0.790363	5.670244	40-44
ATCTC	1367700	0.7176867	5.1502743	35-39
>>END_MODULE