FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | EL589_6KB_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 19974112 |
| Filtered Sequences | 0 |
| Sequence length | 100 |
| %GC | 39 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
![[FAIL]](Icons/error.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 899239 | 4.5020224178176225 | TruSeq Adapter, Index 6 (100% over 50bp) |
| GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG | 35004 | 0.17524683950906053 | No Hit |
| AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA | 23386 | 0.11708155035878441 | No Hit |
| CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC | 22941 | 0.11485366658602895 | No Hit |
| GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT | 22600 | 0.1131464567736478 | No Hit |
| ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC | 21788 | 0.10908119469841764 | No Hit |
| AGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG | 21353 | 0.10690337572954432 | No Hit |
| GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG | 20926 | 0.10476560860377673 | No Hit |
| CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT | 20206 | 0.10116094272426229 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GCCGG | 2592865 | 4.383035 | 5.3619 | 45-49 |
| CCGGC | 2603350 | 4.343514 | 5.3458147 | 75-79 |
| AAAAA | 20553825 | 4.2102356 | 18.091045 | 65-69 |
| CGGGA | 3466625 | 3.8725543 | 4.402192 | 50-54 |
| TCCCG | 3144225 | 3.5024998 | 4.0811305 | 95-96 |
| GGGTT | 4461400 | 3.4510467 | 5.1419673 | 7 |
| TGCCG | 2932300 | 3.3094766 | 28.584976 | 45-49 |
| CCGGG | 1944340 | 3.2867546 | 3.8655791 | 40-44 |
| CGGGC | 1940365 | 3.280035 | 3.9490292 | 55-59 |
| CGTCT | 4238630 | 3.1939812 | 18.646883 | 15-19 |
| ACGGG | 2853205 | 3.1873047 | 3.6007335 | 45-49 |
| CACGT | 4306600 | 3.170258 | 19.366682 | 10-14 |
| CCCGG | 1850865 | 3.088043 | 3.824293 | 95-96 |
| TTTTT | 13245285 | 3.04936 | 3.7748973 | 2 |
| GAAAA | 8409390 | 2.641004 | 8.297013 | 60-64 |
| GCCGT | 2281175 | 2.5745988 | 26.881178 | 45-49 |
| CGCCA | 2312755 | 2.5167928 | 25.622559 | 30-34 |
| TGAAA | 7345835 | 2.3615243 | 7.938578 | 60-64 |
| CAGTC | 3206220 | 2.360225 | 17.774794 | 25-29 |
| ACTCC | 3137970 | 2.279935 | 18.226395 | 20-24 |
| ACGCC | 2061965 | 2.2438772 | 25.389091 | 30-34 |
| GTCTG | 2888620 | 2.205381 | 18.615868 | 15-19 |
| CACGC | 1972640 | 2.1466718 | 24.96991 | 30-34 |
| GGAAG | 2898410 | 2.1396663 | 88.693756 | 5 |
| ACGTC | 2837220 | 2.0885897 | 18.104824 | 15-19 |
| GCACA | 2844375 | 2.0455036 | 17.85775 | 10-14 |
| CCGTC | 1831565 | 2.0402665 | 24.88376 | 50-54 |
| ACACG | 2834770 | 2.0385962 | 17.890406 | 10-14 |
| GCCAA | 2817290 | 2.0260258 | 17.322302 | 30-34 |
| CTGAA | 3969530 | 1.9310557 | 12.521481 | 15-19 |
| CACAC | 2702855 | 1.9184465 | 17.494728 | 10-14 |
| TCCAG | 2588665 | 1.9056184 | 17.221544 | 25-29 |
| GCTTG | 2468640 | 1.8847378 | 17.485756 | 55-59 |
| TCACG | 2532025 | 1.8639234 | 16.9415 | 30-34 |
| CGGAA | 2534635 | 1.8467803 | 86.946 | 4 |
| AACTC | 3800585 | 1.8248187 | 12.305754 | 20-24 |
| CCAGT | 2438210 | 1.7948625 | 17.40675 | 25-29 |
| AGCAC | 2446030 | 1.7590377 | 17.483707 | 10-14 |
| CTCCA | 2390835 | 1.737094 | 17.38174 | 20-24 |
| AGTCA | 3523565 | 1.7141073 | 11.868094 | 25-29 |
| TCTGC | 2251010 | 1.6962281 | 17.137133 | 55-59 |
| TCGGA | 2272200 | 1.6947012 | 88.88863 | 3 |
| GAGCA | 2291185 | 1.6693983 | 86.89918 | 9 |
| GTCAC | 2264380 | 1.6668994 | 17.133942 | 25-29 |
| AGAGC | 2278275 | 1.6599919 | 86.98161 | 8 |
| CTTCT | 3297965 | 1.6592386 | 12.108747 | 50-54 |
| GATCG | 2190820 | 1.6340044 | 88.455376 | 1 |
| ATCGG | 2156425 | 1.6083513 | 88.783134 | 2 |
| GAAGA | 3242700 | 1.5613568 | 58.03084 | 6 |
| CGTAT | 3098475 | 1.5429448 | 12.672284 | 40-44 |
| AAGAG | 3202670 | 1.5420824 | 57.963028 | 7 |
| CCAAT | 3202620 | 1.5377109 | 12.794116 | 35-39 |
| ATGCC | 2073590 | 1.5264516 | 17.787405 | 45-49 |
| TCTCG | 2008325 | 1.513355 | 18.19742 | 40-44 |
| CTGCT | 2001430 | 1.5081594 | 17.147858 | 55-59 |
| CTCGT | 1953005 | 1.4716692 | 18.305803 | 40-44 |
| TTCTG | 2823310 | 1.4391556 | 11.694255 | 55-59 |
| TCTTC | 2819120 | 1.4183267 | 11.833102 | 50-54 |
| GAACT | 2828765 | 1.3761082 | 11.960787 | 20-24 |
| TGAAC | 2803540 | 1.3638369 | 11.736129 | 20-24 |
| TTGAA | 4113755 | 1.3537437 | 7.132394 | 60-64 |
| TCTGA | 2700445 | 1.3447384 | 12.132683 | 15-19 |
| TGCTT | 2616990 | 1.3339857 | 11.93179 | 55-59 |
| GTCTT | 2504100 | 1.2764411 | 11.859295 | 50-54 |
| TCGTA | 2417580 | 1.2038803 | 12.354178 | 40-44 |
| TATGC | 2332925 | 1.1617246 | 12.009756 | 45-49 |
| CTTGA | 2267225 | 1.1290082 | 9.795104 | 60-64 |
| GTATG | 2141500 | 1.0804555 | 11.809206 | 45-49 |
| ATCTC | 2099920 | 1.0320928 | 10.111969 | 40-44 |
| AATAT | 4759445 | 1.0215591 | 5.3326287 | 35-39 |
| ATATC | 2713860 | 0.88145286 | 7.403506 | 35-39 |
| CAATA | 2777075 | 0.8811553 | 7.29661 | 35-39 |
| TATCT | 2538370 | 0.84394324 | 7.461992 | 35-39 |