##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL589_6KB_1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	19974112	
Filtered Sequences	0	
Sequence length	100	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.183145663747155	34.0	31.0	34.0	30.0	34.0
2	32.48252107527984	34.0	31.0	34.0	31.0	34.0
3	32.57942951356235	34.0	31.0	34.0	31.0	34.0
4	35.82232676977079	37.0	35.0	37.0	35.0	37.0
5	35.89095675442292	37.0	35.0	37.0	35.0	37.0
6	35.928403275199415	37.0	36.0	37.0	35.0	37.0
7	35.97842322101729	37.0	36.0	37.0	35.0	37.0
8	36.04058748644245	37.0	36.0	37.0	35.0	37.0
9	37.79152344795103	39.0	38.0	39.0	35.0	39.0
10-14	38.07973850351895	39.4	38.2	39.4	35.2	39.4
15-19	39.11581688337384	41.0	39.0	41.0	35.8	41.0
20-24	38.878895382182705	41.0	39.0	41.0	34.8	41.0
25-29	37.61913976451118	40.6	37.8	41.0	28.4	41.0
30-34	35.941601979602396	40.0	35.4	41.0	22.8	41.0
35-39	36.87936183596047	40.0	36.2	41.0	28.8	41.0
40-44	35.54631975629254	40.0	34.6	41.0	21.4	41.0
45-49	37.5613649507923	40.0	37.2	41.0	32.2	41.0
50-54	37.1919097179389	39.6	36.4	41.0	31.6	41.0
55-59	36.50762336768714	39.0	35.2	41.0	30.8	41.0
60-64	35.44456907020447	37.4	34.8	40.0	28.8	41.0
65-69	34.422229453805	36.2	34.0	39.0	27.8	41.0
70-74	32.61485899348116	35.0	33.4	37.4	21.6	39.6
75-79	31.614348933259215	34.8	32.4	36.2	20.4	37.8
80-84	31.518744653078944	35.0	33.0	35.4	20.6	36.8
85-89	31.04864706876581	35.0	33.0	35.0	18.0	36.0
90-94	30.62757419203417	35.0	32.8	35.0	12.8	36.0
95-99	30.191383657005627	35.0	32.0	35.0	2.0	35.0
100	29.03544528037091	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12443.0
3	1227.0
4	3972.0
5	6260.0
6	10264.0
7	14824.0
8	19185.0
9	23361.0
10	25423.0
11	27865.0
12	31603.0
13	36587.0
14	42168.0
15	46448.0
16	51608.0
17	57716.0
18	65101.0
19	73505.0
20	83452.0
21	96634.0
22	115015.0
23	140571.0
24	184382.0
25	226987.0
26	251747.0
27	347705.0
28	295938.0
29	269545.0
30	335760.0
31	430383.0
32	568127.0
33	814762.0
34	1314859.0
35	1986829.0
36	2416597.0
37	3979265.0
38	4552022.0
39	1006246.0
40	7726.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	25.85601712467678	31.520759586845127	24.405961467974034	18.217261820504067
2	16.80856786299382	36.11342556410432	29.05113579868388	18.026870774217986
3	17.753103674224	28.374440299292065	36.20680073783668	17.66565528864726
4	18.169398332583352	28.303407267847447	29.740598377121497	23.786596022447704
5	24.094638099556065	28.669404677414445	29.456433407402542	17.779523815626945
6	23.518006707882684	29.13980856821069	28.520742248766805	18.821442475139822
7	18.71464924197882	35.33360081289221	28.397637902501	17.554112042627978
8	18.07851481201322	35.09004239542115	28.590117067154647	18.241325725410984
9	24.57595611759862	28.797380329097983	28.413147978743687	18.21351557455971
10-14	19.204720895293377	31.350829275513735	28.81388214671657	20.63056768247632
15-19	19.732545753732783	29.73778153197129	29.834716023363843	20.69495669093208
20-24	19.67938785979685	30.904694795920324	29.906633847884578	19.509283496398243
25-29	19.988673862776903	29.614675903241956	29.398987096104616	20.997663137876525
30-34	19.777517865815796	29.880698520225735	29.443565082826993	20.89821853113148
35-39	18.680516143355593	30.75526403386145	29.566161684175217	20.998058138607735
40-44	18.88095400587405	29.310069989427596	30.897100853956154	20.911875150742198
45-49	21.327048933174165	29.351433048794107	30.826624799949627	18.494893218082105
50-54	19.943039206870477	28.38866059616641	29.597986488034593	22.07031370892852
55-59	19.915209780735722	28.324451677033636	31.958952289439495	19.801386252791144
60-64	20.37696263666647	29.1259740234379	30.53146294929232	19.965600390603306
65-69	18.967774384037796	33.87750668180111	28.522270307103692	18.632448627057403
70-74	19.447160968076776	32.49810760919139	28.686892866817203	19.36783855591463
75-79	19.512471498525347	31.2626785814592	29.431220520418815	19.79362939959664
80-84	20.01501603521293	30.398591867417473	29.546478183964002	20.039913913405595
85-89	19.64178004386756	30.36609987028634	29.558154817912097	20.433965267934003
90-94	19.810789509977138	30.273044469930205	29.53273906669873	20.383426953393922
95-99	19.927590043811268	30.120134517975917	29.40202906491902	20.550246373293792
100	20.441857507762645	30.330642149289123	29.333343680190012	19.894156662758224
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.073278945180846
2	34.83543863721181
3	35.41875896287126
4	41.95599435503106
5	41.87416191518301
6	42.3394491830225
7	36.268761284606796
8	36.319840537424206
9	42.78947169215833
10-14	39.835288577769695
15-19	40.42750244466487
20-24	39.1886713561951
25-29	40.986337000653435
30-34	40.67573639694728
35-39	39.67857428196333
40-44	39.79282915661625
45-49	39.821942151256266
50-54	42.013352915799
55-59	39.71659603352687
60-64	40.342563027269776
65-69	37.600223011095196
70-74	38.81499952399141
75-79	39.30610089812199
80-84	40.05492994861852
85-89	40.07574531180156
90-94	40.19421646337106
95-99	40.47783641710506
100	40.336014170520876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	152.0
2	69.0
3	52.0
4	49.5
5	67.5
6	83.0
7	57.0
8	124.0
9	204.5
10	268.5
11	346.0
12	620.5
13	1124.0
14	1628.0
15	2241.0
16	4451.5
17	6949.5
18	10094.0
19	14616.5
20	21331.5
21	32434.0
22	45736.5
23	63698.0
24	92508.5
25	138242.0
26	200209.5
27	274463.0
28	360050.0
29	451318.5
30	546179.5
31	615499.0
32	658917.5
33	703102.0
34	740500.5
35	774589.0
36	816115.5
37	864075.0
38	875477.5
39	843908.0
40	806979.0
41	802143.5
42	849113.0
43	923872.5
44	989824.0
45	1008973.5
46	971670.0
47	880674.0
48	761715.5
49	655059.5
50	558498.0
51	456266.0
52	328377.5
53	215545.5
54	146806.5
55	103005.0
56	80233.5
57	64080.5
58	47394.0
59	29339.5
60	19595.0
61	15300.5
62	12171.5
63	9988.5
64	8737.0
65	7914.5
66	8001.0
67	7984.5
68	6682.5
69	5894.0
70	5766.5
71	5645.5
72	4616.5
73	3269.0
74	2678.5
75	2351.0
76	2052.5
77	1683.0
78	1115.5
79	558.5
80	215.0
81	123.0
82	100.5
83	78.5
84	54.5
85	50.0
86	50.0
87	58.0
88	85.0
89	53.5
90	3.5
91	2.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08484482313907121
2	0.0029538234290465578
3	2.202851370814382E-4
4	0.001101425685407191
5	0.0
6	0.0
7	0.0
8	1.051360881525046E-4
9	0.0
10-14	1.2015552931714811E-5
15-19	2.002592155285802E-5
20-24	1.061373842301475E-4
25-29	2.392938419490188
30-34	2.4792521439751614
35-39	0.0019515260553260138
40-44	2.1983675669786975
45-49	0.00571940319549625
50-54	0.027533639543024492
55-59	0.039757462058889026
60-64	0.014794149547173863
65-69	0.008274710785640934
70-74	0.029963785123463812
75-79	0.01565926935825733
80-84	0.010658796746508681
85-89	0.00907775023991054
90-94	0.017874136282003423
95-99	0.005847569093434542
100	0.0012616330578300553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.9974112E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	96.99926720205782
#Duplication Level	Relative count
1	100.0
2	8.44873209687563
3	2.815848492399701
4	1.5782041697815432
5	1.044225315344179
6	0.8780985606303324
7	0.6989189894746834
8	0.6039894153524854
9	0.5316056150843094
10++	120.72431265055238
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	899239	4.5020224178176225	TruSeq Adapter, Index 6 (100% over 50bp)
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG	35004	0.17524683950906053	No Hit
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA	23386	0.11708155035878441	No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC	22941	0.11485366658602895	No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT	22600	0.1131464567736478	No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC	21788	0.10908119469841764	No Hit
AGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG	21353	0.10690337572954432	No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG	20926	0.10476560860377673	No Hit
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT	20206	0.10116094272426229	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCCGG	2592865	4.383035	5.3619	45-49
CCGGC	2603350	4.343514	5.3458147	75-79
AAAAA	20553825	4.2102356	18.091045	65-69
CGGGA	3466625	3.8725543	4.402192	50-54
TCCCG	3144225	3.5024998	4.0811305	95-96
GGGTT	4461400	3.4510467	5.1419673	7
TGCCG	2932300	3.3094766	28.584976	45-49
CCGGG	1944340	3.2867546	3.8655791	40-44
CGGGC	1940365	3.280035	3.9490292	55-59
CGTCT	4238630	3.1939812	18.646883	15-19
ACGGG	2853205	3.1873047	3.6007335	45-49
CACGT	4306600	3.170258	19.366682	10-14
CCCGG	1850865	3.088043	3.824293	95-96
TTTTT	13245285	3.04936	3.7748973	2
GAAAA	8409390	2.641004	8.297013	60-64
GCCGT	2281175	2.5745988	26.881178	45-49
CGCCA	2312755	2.5167928	25.622559	30-34
TGAAA	7345835	2.3615243	7.938578	60-64
CAGTC	3206220	2.360225	17.774794	25-29
ACTCC	3137970	2.279935	18.226395	20-24
ACGCC	2061965	2.2438772	25.389091	30-34
GTCTG	2888620	2.205381	18.615868	15-19
CACGC	1972640	2.1466718	24.96991	30-34
GGAAG	2898410	2.1396663	88.693756	5
ACGTC	2837220	2.0885897	18.104824	15-19
GCACA	2844375	2.0455036	17.85775	10-14
CCGTC	1831565	2.0402665	24.88376	50-54
ACACG	2834770	2.0385962	17.890406	10-14
GCCAA	2817290	2.0260258	17.322302	30-34
CTGAA	3969530	1.9310557	12.521481	15-19
CACAC	2702855	1.9184465	17.494728	10-14
TCCAG	2588665	1.9056184	17.221544	25-29
GCTTG	2468640	1.8847378	17.485756	55-59
TCACG	2532025	1.8639234	16.9415	30-34
CGGAA	2534635	1.8467803	86.946	4
AACTC	3800585	1.8248187	12.305754	20-24
CCAGT	2438210	1.7948625	17.40675	25-29
AGCAC	2446030	1.7590377	17.483707	10-14
CTCCA	2390835	1.737094	17.38174	20-24
AGTCA	3523565	1.7141073	11.868094	25-29
TCTGC	2251010	1.6962281	17.137133	55-59
TCGGA	2272200	1.6947012	88.88863	3
GAGCA	2291185	1.6693983	86.89918	9
GTCAC	2264380	1.6668994	17.133942	25-29
AGAGC	2278275	1.6599919	86.98161	8
CTTCT	3297965	1.6592386	12.108747	50-54
GATCG	2190820	1.6340044	88.455376	1
ATCGG	2156425	1.6083513	88.783134	2
GAAGA	3242700	1.5613568	58.03084	6
CGTAT	3098475	1.5429448	12.672284	40-44
AAGAG	3202670	1.5420824	57.963028	7
CCAAT	3202620	1.5377109	12.794116	35-39
ATGCC	2073590	1.5264516	17.787405	45-49
TCTCG	2008325	1.513355	18.19742	40-44
CTGCT	2001430	1.5081594	17.147858	55-59
CTCGT	1953005	1.4716692	18.305803	40-44
TTCTG	2823310	1.4391556	11.694255	55-59
TCTTC	2819120	1.4183267	11.833102	50-54
GAACT	2828765	1.3761082	11.960787	20-24
TGAAC	2803540	1.3638369	11.736129	20-24
TTGAA	4113755	1.3537437	7.132394	60-64
TCTGA	2700445	1.3447384	12.132683	15-19
TGCTT	2616990	1.3339857	11.93179	55-59
GTCTT	2504100	1.2764411	11.859295	50-54
TCGTA	2417580	1.2038803	12.354178	40-44
TATGC	2332925	1.1617246	12.009756	45-49
CTTGA	2267225	1.1290082	9.795104	60-64
GTATG	2141500	1.0804555	11.809206	45-49
ATCTC	2099920	1.0320928	10.111969	40-44
AATAT	4759445	1.0215591	5.3326287	35-39
ATATC	2713860	0.88145286	7.403506	35-39
CAATA	2777075	0.8811553	7.29661	35-39
TATCT	2538370	0.84394324	7.461992	35-39
>>END_MODULE