FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | EL387_5KB_2.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 81147111 |
| Filtered Sequences | 0 |
| Sequence length | 100 |
| %GC | 38 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 96003 | 0.11830735415830146 | Illumina Single End PCR Primer 1 (100% over 50bp) |
![[FAIL]](Icons/error.png)
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGGGG | 14837455 | 6.5722547 | 40.189327 | 40-44 |
| GGCCT | 11953275 | 3.6768656 | 3.9559171 | 4 |
| AAAAA | 82654740 | 3.6154723 | 13.761112 | 60-64 |
| AGGCC | 11879440 | 3.5549092 | 4.0897017 | 2 |
| GCGCC | 5654830 | 2.7856603 | 10.535364 | 45-49 |
| GGCGC | 5513450 | 2.6214857 | 9.829565 | 40-44 |
| GGGCG | 4116340 | 1.8890818 | 9.336773 | 40-44 |
| CGGGG | 3957840 | 1.8163426 | 9.127116 | 35-39 |
| GGGGC | 3936365 | 1.8064872 | 11.19496 | 40-44 |
| CGCCG | 3528165 | 1.7380309 | 12.644877 | 45-49 |
| GGGGA | 6233265 | 1.7377169 | 10.560331 | 20-24 |
| GGAAA | 15708235 | 1.7346334 | 5.9534855 | 6 |
| AGGGG | 6051775 | 1.6871209 | 8.099436 | 25-29 |
| GGGCC | 3529215 | 1.6780394 | 6.6467166 | 45-49 |
| GGGAA | 9395745 | 1.6485536 | 10.367991 | 20-24 |
| GGAAG | 9316560 | 1.63466 | 24.726938 | 5 |
| GGCGG | 3417020 | 1.568148 | 8.199258 | 10-14 |
| GCGTC | 4930135 | 1.5165251 | 6.910078 | 10-14 |
| GGTGG | 5238710 | 1.5012261 | 5.576652 | 40-44 |
| CGTCG | 4666880 | 1.435547 | 6.9018693 | 10-14 |
| GAAGA | 12996490 | 1.4351802 | 16.127584 | 6 |
| GAGGG | 5075240 | 1.4148813 | 12.102467 | 9 |
| GCCGT | 4474505 | 1.376372 | 5.2046866 | 45-49 |
| GCCGG | 2887140 | 1.3727514 | 7.391003 | 45-49 |
| GCGGC | 2871935 | 1.3655218 | 5.3952537 | 10-14 |
| GGGAG | 4732595 | 1.3193583 | 11.935926 | 5 |
| GCGGG | 2864155 | 1.3144257 | 7.630532 | 10-14 |
| CGGCG | 2700780 | 1.2841426 | 5.23706 | 10-14 |
| ATCGG | 6506395 | 1.2157806 | 31.290287 | 2 |
| AGAGG | 6927830 | 1.2155397 | 7.9250226 | 8 |
| AAGAG | 10860980 | 1.1993593 | 15.362365 | 7 |
| AAGGG | 6729565 | 1.1807524 | 6.303997 | 9 |
| GAGCG | 4032585 | 1.1647468 | 38.377644 | 9 |
| TCGGG | 3791155 | 1.1255839 | 11.424965 | 3 |
| CGGGA | 3883990 | 1.1218275 | 14.84469 | 4 |
| AGAGC | 6025165 | 1.0952806 | 24.64171 | 8 |
| CGGAA | 5964715 | 1.0842918 | 26.618456 | 4 |
| GGAGA | 5546875 | 0.9732407 | 9.336539 | 6 |
| GATCG | 5174025 | 0.9668147 | 31.000452 | 1 |
| TAGGG | 5340415 | 0.9631752 | 5.1073055 | 15-19 |
| CTCGG | 3080200 | 0.94747937 | 7.806209 | 35-39 |
| GAGAG | 5198915 | 0.9121885 | 8.169644 | 7 |
| TCGGA | 4878205 | 0.9115381 | 25.465324 | 3 |