##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL387_5KB_2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	81147111	
Filtered Sequences	0	
Sequence length	100	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610445231993534	34.0	31.0	34.0	30.0	34.0
2	31.692690895674648	34.0	31.0	34.0	30.0	34.0
3	31.71466880687841	34.0	31.0	34.0	30.0	34.0
4	35.04077920900967	37.0	35.0	37.0	33.0	37.0
5	35.066622926354086	37.0	35.0	37.0	33.0	37.0
6	35.15565209709068	37.0	36.0	37.0	33.0	37.0
7	34.93657185158446	37.0	36.0	37.0	33.0	37.0
8	35.035379546660636	37.0	36.0	37.0	33.0	37.0
9	36.5216815667042	39.0	38.0	39.0	33.0	39.0
10-14	36.80323640850257	39.4	38.2	39.4	33.0	39.4
15-19	37.87607680574112	41.0	39.0	41.0	33.0	41.0
20-24	37.79261976929776	41.0	39.0	41.0	33.4	41.0
25-29	37.58834553456869	40.6	38.2	41.0	32.6	41.0
30-34	37.26109233143248	40.0	38.0	41.0	31.4	41.0
35-39	37.301160473846075	40.0	38.0	41.0	31.6	41.0
40-44	37.13686714737139	40.0	38.0	41.0	31.4	41.0
45-49	36.991469460939896	40.0	38.0	41.0	30.8	41.0
50-54	36.28985044951261	39.6	37.0	40.6	30.2	41.0
55-59	36.19738139537709	39.8	36.2	41.0	29.0	41.0
60-64	35.7531724105372	39.0	35.2	41.0	29.0	41.0
65-69	35.011447088978926	37.4	35.0	40.4	28.2	41.0
70-74	34.24434137895557	36.4	35.0	39.0	28.0	41.0
75-79	33.41709324932098	35.2	34.2	37.4	27.4	39.4
80-84	32.68759746480684	35.0	34.0	36.2	26.0	38.2
85-89	32.13745642281707	35.0	34.0	35.6	25.8	36.8
90-94	31.69768546165495	35.0	34.0	35.0	24.8	36.0
95-99	31.266262689253345	35.0	33.6	35.0	22.6	36.0
100	30.185170252086976	34.0	31.0	35.0	16.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1892014.0
3	493278.0
4	136193.0
5	75630.0
6	160338.0
7	243339.0
8	133280.0
9	88936.0
10	91134.0
11	97358.0
12	95046.0
13	100570.0
14	135380.0
15	163132.0
16	166347.0
17	166012.0
18	168470.0
19	167575.0
20	164126.0
21	166642.0
22	179824.0
23	208514.0
24	250818.0
25	304177.0
26	371794.0
27	456277.0
28	565272.0
29	700510.0
30	876533.0
31	1111352.0
32	1425974.0
33	1881701.0
34	2615555.0
35	3998296.0
36	7704096.0
37	1.8151197E7
38	2.6344795E7
39	9038705.0
40	56921.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.67091562580511	31.195297920178454	27.312851542812776	19.820934911203654
2	18.221292599708903	35.69016473774555	29.016696087378108	17.07184657516744
3	17.73716939503335	31.19328746547791	32.838465626257076	18.231077513231668
4	18.646578804265747	29.412678659625975	30.514742539632746	21.425999996475536
5	21.25045462185339	29.927960097063718	30.38180866352716	18.439776617555736
6	22.081708426397515	30.134613184191572	28.994094726043635	18.78958366336728
7	20.09419768263014	32.27597758957608	29.398857325894344	18.23096740189943
8	17.884055046642388	33.822093801959255	30.02906412774202	18.264787023656332
9	20.679203834413165	30.818901714233395	30.132857139248138	18.369037312105306
10-14	20.15083654884063	30.88738019153383	30.25014527868067	18.71163798094487
15-19	20.32864542028757	29.83194142847317	30.798608317562426	19.04080483367683
20-24	21.180526774550717	30.471551499621125	29.94818411049551	18.39973761533264
25-29	19.87553822354144	32.01373951064275	29.638560006049385	18.47216225976642
30-34	20.92307364304114	30.415091619078183	30.161597257601898	18.500237480278788
35-39	18.699408252487483	30.74969689354681	31.05343318793739	19.49746166602832
40-44	21.652703427990186	29.74084295914978	29.870978015315757	18.735475597544273
45-49	20.0789752877015	29.80306656996385	29.90148382436694	20.216474317967705
50-54	19.595440226089398	30.2660052193213	30.65273565676476	19.48581889782454
55-59	18.52103818421377	31.4590568750228	31.438037385784824	18.581867554978608
60-64	18.28500216129858	33.577375193078225	29.7372488283812	18.400373817241995
65-69	18.455901049408947	33.10009960176548	29.967030079330897	18.476969269494667
70-74	18.75665834017885	32.13904698979901	30.28354072455459	18.82075394546754
75-79	19.03895501278242	31.478160159888947	30.508275202295575	18.974609625033057
80-84	19.098391561875502	31.227728585544252	30.55312640572827	19.120753446851975
85-89	19.24155205459101	31.064933210832624	30.53511461638068	19.158400118195683
90-94	19.29347820862754	30.961402531415615	30.517744220157876	19.227375039798964
95-99	19.457556570315383	30.800959120147255	30.57490680598662	19.166577503550748
100	19.52069053782475	30.973186036428512	30.318311122493547	19.187812303253192
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.491850537008766
2	35.293139174876345
3	35.96824690826502
4	40.07257880074129
5	39.69023123940913
6	40.8712920897648
7	38.325165084529566
8	36.14884207029872
9	39.04824114651847
10-14	38.86247452978551
15-19	39.3694502539644
20-24	39.580264389883354
25-29	38.34770048330786
30-34	39.42331112331992
35-39	38.19686991851581
40-44	40.388179025534455
45-49	40.295449605669205
50-54	39.08125912391394
55-59	37.10290573919238
60-64	36.68537597854058
65-69	36.93287031890362
70-74	37.57741228564639
75-79	38.01356463781547
80-84	38.21914500872748
85-89	38.3999521727867
90-94	38.520853248426505
95-99	38.62413407386613
100	38.708502841077944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1416.0
1	1383.5
2	1564.5
3	1940.5
4	2286.5
5	2625.5
6	3094.0
7	3675.5
8	4228.0
9	5009.5
10	5684.5
11	6823.5
12	9221.0
13	12031.5
14	15341.0
15	22248.5
16	32748.5
17	48892.0
18	73604.5
19	108783.0
20	160235.5
21	230403.5
22	328036.0
23	464852.0
24	635942.5
25	842992.5
26	1092317.0
27	1383659.5
28	1715445.5
29	2085361.0
30	2466267.5
31	2841746.5
32	3203163.5
33	3547352.5
34	3856081.5
35	4051680.5
36	4105705.0
37	4047633.5
38	3883502.0
39	3645706.5
40	3375502.5
41	3091108.0
42	2839318.5
43	2638459.0
44	2501248.0
45	2436868.0
46	2433066.0
47	2472629.0
48	2473183.5
49	2366545.5
50	2156269.5
51	1856318.0
52	1497552.5
53	1147775.5
54	849377.0
55	613856.5
56	435246.5
57	298879.5
58	204145.5
59	137020.0
60	90024.5
61	61610.0
62	43217.0
63	31871.5
64	24743.0
65	18975.0
66	15789.0
67	13338.5
68	11692.0
69	9605.0
70	7988.5
71	7684.5
72	6530.0
73	4995.5
74	4106.0
75	3631.0
76	3391.5
77	2923.0
78	2249.0
79	1660.5
80	1339.0
81	1063.5
82	809.0
83	677.0
84	583.0
85	469.5
86	377.0
87	279.0
88	230.0
89	193.5
90	148.5
91	118.5
92	94.0
93	75.0
94	65.5
95	58.0
96	45.5
97	38.5
98	29.0
99	19.0
100	18.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02583949045333234
2	2.4030430362456158E-4
3	0.0015391798729593711
4	0.0
5	0.0
6	1.9224344289964928E-4
7	0.002483144470787136
8	0.0
9	0.0011411373597761231
10-14	0.004200765693309772
15-19	0.012653069066131017
20-24	0.003370914831459619
25-29	0.008610041582379933
30-34	0.00435135639024783
35-39	0.019161495472093885
40-44	0.03602099894844069
45-49	0.03951613261992778
50-54	0.027588166385861847
55-59	0.03581150288887056
60-64	0.03200754737898186
65-69	0.010832425075490365
70-74	0.0037144883691546332
75-79	0.001959650787814245
80-84	0.002334525501468561
85-89	0.0020661240792663586
90-94	0.0037504723982102087
95-99	0.0022472765543064127
100	7.665091120742425E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8.1147111E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	46.44601428221543
#Duplication Level	Relative count
1	100.0
2	36.633308224828724
3	11.577710821765487
4	4.204777789734209
5	2.1476738763944185
6	1.4051795258079558
7	1.0527637289716274
8	0.8653466767100685
9	0.7684995875031385
10++	20.69120126259909
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	96003	0.11830735415830146	Illumina Single End PCR Primer 1 (100% over 50bp)
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	14837455	6.5722547	40.189327	40-44
GGCCT	11953275	3.6768656	3.9559171	4
AAAAA	82654740	3.6154723	13.761112	60-64
AGGCC	11879440	3.5549092	4.0897017	2
GCGCC	5654830	2.7856603	10.535364	45-49
GGCGC	5513450	2.6214857	9.829565	40-44
GGGCG	4116340	1.8890818	9.336773	40-44
CGGGG	3957840	1.8163426	9.127116	35-39
GGGGC	3936365	1.8064872	11.19496	40-44
CGCCG	3528165	1.7380309	12.644877	45-49
GGGGA	6233265	1.7377169	10.560331	20-24
GGAAA	15708235	1.7346334	5.9534855	6
AGGGG	6051775	1.6871209	8.099436	25-29
GGGCC	3529215	1.6780394	6.6467166	45-49
GGGAA	9395745	1.6485536	10.367991	20-24
GGAAG	9316560	1.63466	24.726938	5
GGCGG	3417020	1.568148	8.199258	10-14
GCGTC	4930135	1.5165251	6.910078	10-14
GGTGG	5238710	1.5012261	5.576652	40-44
CGTCG	4666880	1.435547	6.9018693	10-14
GAAGA	12996490	1.4351802	16.127584	6
GAGGG	5075240	1.4148813	12.102467	9
GCCGT	4474505	1.376372	5.2046866	45-49
GCCGG	2887140	1.3727514	7.391003	45-49
GCGGC	2871935	1.3655218	5.3952537	10-14
GGGAG	4732595	1.3193583	11.935926	5
GCGGG	2864155	1.3144257	7.630532	10-14
CGGCG	2700780	1.2841426	5.23706	10-14
ATCGG	6506395	1.2157806	31.290287	2
AGAGG	6927830	1.2155397	7.9250226	8
AAGAG	10860980	1.1993593	15.362365	7
AAGGG	6729565	1.1807524	6.303997	9
GAGCG	4032585	1.1647468	38.377644	9
TCGGG	3791155	1.1255839	11.424965	3
CGGGA	3883990	1.1218275	14.84469	4
AGAGC	6025165	1.0952806	24.64171	8
CGGAA	5964715	1.0842918	26.618456	4
GGAGA	5546875	0.9732407	9.336539	6
GATCG	5174025	0.9668147	31.000452	1
TAGGG	5340415	0.9631752	5.1073055	15-19
CTCGG	3080200	0.94747937	7.806209	35-39
GAGAG	5198915	0.9121885	8.169644	7
TCGGA	4878205	0.9115381	25.465324	3
>>END_MODULE