##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL387_5KB_1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	81147111	
Filtered Sequences	0	
Sequence length	100	
%GC	38	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56308290507101	34.0	31.0	34.0	31.0	34.0
2	32.83516263444055	34.0	31.0	34.0	31.0	34.0
3	32.916829238689715	34.0	31.0	34.0	31.0	34.0
4	36.14314187969058	37.0	37.0	37.0	35.0	37.0
5	36.18748393889217	37.0	37.0	37.0	35.0	37.0
6	36.21668923000845	37.0	37.0	37.0	35.0	37.0
7	36.21949737680741	37.0	37.0	37.0	35.0	37.0
8	36.251692854475124	37.0	37.0	37.0	35.0	37.0
9	38.023613607143695	39.0	39.0	39.0	35.0	39.0
10-14	38.30812835961591	39.4	39.0	39.4	35.2	39.4
15-19	39.40665202486383	41.0	39.0	41.0	36.0	41.0
20-24	39.18169629477013	41.0	39.0	41.0	36.0	41.0
25-29	37.97571931796808	40.8	38.2	41.0	29.2	41.0
30-34	36.386471193041	40.0	36.0	41.0	23.4	41.0
35-39	37.33713037793792	40.0	36.6	41.0	29.6	41.0
40-44	36.14069149547419	40.0	35.6	41.0	22.6	41.0
45-49	38.30182337606573	40.0	38.0	41.0	33.8	41.0
50-54	38.06839599255727	40.0	37.6	41.0	33.4	41.0
55-59	37.51680329814822	39.6	36.6	41.0	32.2	41.0
60-64	36.69406787630431	38.8	35.4	40.6	31.4	41.0
65-69	36.026530211285035	37.4	35.0	39.8	31.0	41.0
70-74	34.43110953389333	36.2	34.6	38.8	28.6	40.8
75-79	33.31885075736091	35.0	33.8	37.0	28.0	39.2
80-84	33.09116457885974	35.0	34.0	36.0	29.0	37.8
85-89	32.52688544142995	35.0	34.0	35.4	28.4	36.6
90-94	32.089060341285595	35.0	34.0	35.0	26.8	36.0
95-99	31.71327174173829	35.0	33.8	35.0	25.6	36.0
100	30.576920600414226	34.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7055.0
3	838.0
4	2321.0
5	3969.0
6	6539.0
7	11192.0
8	20740.0
9	33673.0
10	42120.0
11	49891.0
12	58893.0
13	74091.0
14	91533.0
15	109148.0
16	124948.0
17	157731.0
18	162319.0
19	189665.0
20	216053.0
21	244526.0
22	291498.0
23	366159.0
24	488226.0
25	618704.0
26	739065.0
27	1037963.0
28	980953.0
29	897754.0
30	1125453.0
31	1451279.0
32	1928838.0
33	2762137.0
34	4625773.0
35	7717571.0
36	9165217.0
37	1.5589903E7
38	2.2299956E7
39	7412868.0
40	40549.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.59107001429713	31.15191259327065	27.06077940400224	19.196237988429978
2	17.385537510325083	35.333660699468695	30.134606093037497	17.146195697168725
3	17.41076475633985	29.566848121083293	34.972744128125726	18.049642994451126
4	18.564146104399484	29.19702048280358	30.285494569324513	21.953338843472423
5	22.20776165401625	29.877953387644325	30.133659348636577	17.780625609702852
6	22.124740830268127	30.181755946924593	29.223447030665085	18.470056192142195
7	18.297352569951627	34.14278913761945	29.529010589175503	18.030847703253418
8	17.677817796757424	34.01266875351183	29.986605058677917	18.322908391052824
9	21.991698509143472	29.77907617684627	29.92997125923559	18.299254054774668
10-14	18.8413028591823	31.502112235607747	29.85959912791859	19.79698577729136
15-19	18.991257680364736	30.49798451060171	30.647550933379	19.86320687565455
20-24	19.098949635744564	31.20892787730918	30.498777507655145	19.193344979291115
25-29	19.35664960886008	30.32528311206094	30.256371697506975	20.061695581572
30-34	18.722210807565656	30.395726306080718	30.246881857657566	20.635181028696064
35-39	19.305215412807865	31.250331117816703	30.294686310499873	19.14976715887556
40-44	18.79931850780058	30.314413648414323	30.934891551519467	19.95137629226563
45-49	20.107020589823634	30.483896455787313	30.990091461992485	18.418991492396568
50-54	19.17638908541175	29.780683348364672	30.35589576723034	20.68703179899324
55-59	19.193325020028112	29.799432668007224	31.783386182747037	19.223856129217626
60-64	19.275922353280453	30.437554045286998	31.12955705170216	19.156966549730388
65-69	18.424700291328108	33.36298532829527	29.77644624864284	18.43586813173378
70-74	18.73411665750129	32.67660829654456	29.83717977422442	18.752095271729733
75-79	18.814886705435867	31.619331006008505	30.495154812767268	19.07062747578836
80-84	18.951425538447385	31.179540825545633	30.542084323182916	19.32694931282406
85-89	19.13121408637444	31.061451293639603	30.451106929146643	19.35622769083932
90-94	19.15823984126935	30.871786268410535	30.50459650870722	19.465377381612896
95-99	19.299379115008513	30.7889216953375	30.519181336435718	19.392517853218262
100	19.29043838371388	31.00515991507319	30.27268974333863	19.431711957874302
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.78730800272711
2	34.53173320749381
3	35.46040775079098
4	40.51748494787191
5	39.9883872637191
6	40.594797022410326
7	36.32820027320504
8	36.00072618781025
9	40.29095256391814
10-14	38.63828863647366
15-19	38.85446455601929
20-24	38.29229461503568
25-29	39.418345190432085
30-34	39.35739183626172
35-39	38.45498257168342
40-44	38.75069480006621
45-49	38.5260120822202
50-54	39.86342088440499
55-59	38.417181149245735
60-64	38.43288890301084
65-69	36.86056842306189
70-74	37.48621192923102
75-79	37.88551418122423
80-84	38.278374851271444
85-89	38.48744177721376
90-94	38.623617222882245
95-99	38.69189696822678
100	38.72215034158818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	623.0
1	424.0
2	200.5
3	285.5
4	298.0
5	215.5
6	318.5
7	441.0
8	623.5
9	885.0
10	1341.5
11	2570.5
12	4363.0
13	6989.5
14	10707.0
15	16036.0
16	26511.5
17	42668.5
18	66347.0
19	102422.0
20	152052.5
21	221396.5
22	321944.0
23	456359.5
24	635359.0
25	852735.5
26	1104336.5
27	1393773.0
28	1720946.5
29	2090601.5
30	2480226.0
31	2855980.0
32	3219319.5
33	3591769.0
34	3897084.5
35	4074204.0
36	4137592.0
37	4078237.0
38	3904389.0
39	3678908.0
40	3431223.5
41	3183301.0
42	2974514.0
43	2796919.5
44	2663268.5
45	2580760.5
46	2544876.5
47	2534327.5
48	2484136.0
49	2337647.0
50	2086572.5
51	1750617.0
52	1380914.5
53	1047755.0
54	744798.5
55	497737.5
56	330731.5
57	211982.5
58	133671.5
59	82124.5
60	50535.5
61	31099.0
62	20168.5
63	14506.5
64	11445.0
65	10367.5
66	8948.5
67	7666.0
68	7987.0
69	7467.5
70	5228.0
71	4027.0
72	3805.0
73	3447.5
74	2969.0
75	2540.0
76	2064.0
77	1616.0
78	930.0
79	388.0
80	241.5
81	194.0
82	142.0
83	90.0
84	61.5
85	36.5
86	26.0
87	16.0
88	8.0
89	7.0
90	11.0
91	10.0
92	4.0
93	1.5
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08712448185616861
2	0.0030721980971078564
3	2.3783964410020711E-4
4	0.0011633192954953134
5	0.0
6	0.0
7	0.0
8	7.270745596845709E-5
9	0.0
10-14	1.010510404985336E-5
15-19	2.5632459053286565E-5
20-24	1.2298651026528844E-4
25-29	2.39025588970136
30-34	2.474661605636213
35-39	0.0018765917618434992
40-44	2.190485623080284
45-49	0.005799836792710957
50-54	0.028121025775027283
55-59	0.040819444576406426
60-64	0.015537213641530627
65-69	0.00860535872928366
70-74	0.031023408830907116
75-79	0.01621326974906106
80-84	0.011122761967459323
85-89	0.009331940357063358
90-94	0.018553217501483203
95-99	0.0060800685806300615
100	0.0012421884002746567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	8.1147111E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	43.61474083233473
#Duplication Level	Relative count
1	100.0
2	32.297805324121114
3	9.286730339361918
4	3.27338616812301
5	1.7772471719840142
6	1.2590598116913907
7	1.0041996884102147
8	0.8153830522251575
9	0.6909164803901646
10++	18.937546569125516
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC	140570	0.17322859466925444	TruSeq Adapter, Index 3 (97% over 37bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGCCT	11594180	3.622231	3.9844296	8
AGGCC	11704330	3.5917497	4.051625	7
CACGT	18030625	3.476278	14.346555	10-14
AAAAA	69551200	3.1664722	11.818868	65-69
GCCGT	6656770	2.079695	18.124994	45-49
CGTCT	10154585	1.9931614	12.321796	15-19
GGAAG	10258720	1.9872907	59.489246	5
ACACG	10397240	1.9689978	12.6232605	10-14
GAAAA	26555825	1.9591299	5.5232196	60-64
ACGTC	9949940	1.9183338	12.616966	15-19
TGCCG	6079735	1.8994188	18.31643	45-49
CAGTC	9812265	1.8917903	12.44544	25-29
CCGTC	6118165	1.8898909	17.141888	50-54
GCACA	9948580	1.8840315	12.6116085	10-14
GTCTG	8821020	1.751135	12.845831	15-19
TGAAA	23065350	1.7323679	5.233924	60-64
GAAGA	14090665	1.6844856	37.292507	6
CACAC	8813130	1.6502007	12.239581	10-14
CACCA	8700335	1.6290805	10.829226	30-34
CTCCA	8536930	1.6273646	12.197925	20-24
TCCAG	8317860	1.6036712	12.011145	25-29
AGCAC	8377985	1.5865972	12.261164	10-14
TCTTC	12811055	1.5798266	7.715687	50-54
GTCAC	8144910	1.5703264	12.048628	25-29
ATCGG	8026510	1.5651321	59.3844	2
CCAGT	8112745	1.5641253	12.130425	25-29
CTGCT	7898310	1.5502955	11.362324	55-59
CTTCT	12498470	1.5412796	7.728478	50-54
AGAGC	7996340	1.5315772	58.44961	8
ACTCC	7845610	1.4955808	12.186575	20-24
GCTTG	7455330	1.4800203	11.260562	55-59
CGGAA	7683700	1.4716957	58.0372	4
GAGCA	7604540	1.456534	58.377415	9
CTGAA	12098560	1.45588	8.206713	15-19
TCTGC	7356740	1.4439951	11.190945	55-59
TGCTT	11382420	1.4196447	7.652251	55-59
TTCTG	11252155	1.4033978	7.455862	55-59
CCAGA	7176105	1.358989	10.747791	30-34
GATCG	6924980	1.3503388	59.092197	1
AGTCA	11107395	1.336608	7.93325	25-29
TCGGA	6840840	1.3339322	58.997757	3
TCACC	6996025	1.3336273	10.495416	30-34
ACCAG	7036685	1.3325859	10.752249	30-34
ATGCC	6866005	1.3237556	11.560462	45-49
AACTC	10992635	1.3078959	8.037303	20-24
GAACT	10829880	1.3032132	8.066188	20-24
TGAAC	10821345	1.3021864	7.9277186	20-24
AAGAG	10842490	1.2961787	36.819817	7
TCTGA	10319860	1.2642772	8.118413	15-19
CTCGT	6427165	1.2615362	11.976622	40-44
TCGTA	9964240	1.2207105	7.954684	40-44
TCTCG	6086710	1.1947112	11.83411	40-44
GTCTT	9258435	1.1547358	7.420643	50-54
TCATC	9269615	1.1228186	6.6409526	35-39
CTTGA	9054800	1.1092957	6.7135553	60-64
AGATC	8427925	1.0141741	7.846209	35-39
CATCT	8359790	1.0126125	6.652397	35-39
TATGC	8129065	0.9958847	7.4326353	45-49
CAGAT	8267725	0.9948966	6.8818064	30-34
GTATG	7936395	0.98335946	7.3825326	45-49
ATCTC	7671680	0.92926246	6.4436584	40-44
GATCA	7467450	0.8985954	6.5926514	35-39
CGTAT	7312125	0.8958021	7.4932046	40-44
>>END_MODULE