FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | EL387_5KB_1.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 81147111 |
| Filtered Sequences | 0 |
| Sequence length | 100 |
| %GC | 38 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC | 140570 | 0.17322859466925444 | TruSeq Adapter, Index 3 (97% over 37bp) |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGCCT | 11594180 | 3.622231 | 3.9844296 | 8 |
| AGGCC | 11704330 | 3.5917497 | 4.051625 | 7 |
| CACGT | 18030625 | 3.476278 | 14.346555 | 10-14 |
| AAAAA | 69551200 | 3.1664722 | 11.818868 | 65-69 |
| GCCGT | 6656770 | 2.079695 | 18.124994 | 45-49 |
| CGTCT | 10154585 | 1.9931614 | 12.321796 | 15-19 |
| GGAAG | 10258720 | 1.9872907 | 59.489246 | 5 |
| ACACG | 10397240 | 1.9689978 | 12.6232605 | 10-14 |
| GAAAA | 26555825 | 1.9591299 | 5.5232196 | 60-64 |
| ACGTC | 9949940 | 1.9183338 | 12.616966 | 15-19 |
| TGCCG | 6079735 | 1.8994188 | 18.31643 | 45-49 |
| CAGTC | 9812265 | 1.8917903 | 12.44544 | 25-29 |
| CCGTC | 6118165 | 1.8898909 | 17.141888 | 50-54 |
| GCACA | 9948580 | 1.8840315 | 12.6116085 | 10-14 |
| GTCTG | 8821020 | 1.751135 | 12.845831 | 15-19 |
| TGAAA | 23065350 | 1.7323679 | 5.233924 | 60-64 |
| GAAGA | 14090665 | 1.6844856 | 37.292507 | 6 |
| CACAC | 8813130 | 1.6502007 | 12.239581 | 10-14 |
| CACCA | 8700335 | 1.6290805 | 10.829226 | 30-34 |
| CTCCA | 8536930 | 1.6273646 | 12.197925 | 20-24 |
| TCCAG | 8317860 | 1.6036712 | 12.011145 | 25-29 |
| AGCAC | 8377985 | 1.5865972 | 12.261164 | 10-14 |
| TCTTC | 12811055 | 1.5798266 | 7.715687 | 50-54 |
| GTCAC | 8144910 | 1.5703264 | 12.048628 | 25-29 |
| ATCGG | 8026510 | 1.5651321 | 59.3844 | 2 |
| CCAGT | 8112745 | 1.5641253 | 12.130425 | 25-29 |
| CTGCT | 7898310 | 1.5502955 | 11.362324 | 55-59 |
| CTTCT | 12498470 | 1.5412796 | 7.728478 | 50-54 |
| AGAGC | 7996340 | 1.5315772 | 58.44961 | 8 |
| ACTCC | 7845610 | 1.4955808 | 12.186575 | 20-24 |
| GCTTG | 7455330 | 1.4800203 | 11.260562 | 55-59 |
| CGGAA | 7683700 | 1.4716957 | 58.0372 | 4 |
| GAGCA | 7604540 | 1.456534 | 58.377415 | 9 |
| CTGAA | 12098560 | 1.45588 | 8.206713 | 15-19 |
| TCTGC | 7356740 | 1.4439951 | 11.190945 | 55-59 |
| TGCTT | 11382420 | 1.4196447 | 7.652251 | 55-59 |
| TTCTG | 11252155 | 1.4033978 | 7.455862 | 55-59 |
| CCAGA | 7176105 | 1.358989 | 10.747791 | 30-34 |
| GATCG | 6924980 | 1.3503388 | 59.092197 | 1 |
| AGTCA | 11107395 | 1.336608 | 7.93325 | 25-29 |
| TCGGA | 6840840 | 1.3339322 | 58.997757 | 3 |
| TCACC | 6996025 | 1.3336273 | 10.495416 | 30-34 |
| ACCAG | 7036685 | 1.3325859 | 10.752249 | 30-34 |
| ATGCC | 6866005 | 1.3237556 | 11.560462 | 45-49 |
| AACTC | 10992635 | 1.3078959 | 8.037303 | 20-24 |
| GAACT | 10829880 | 1.3032132 | 8.066188 | 20-24 |
| TGAAC | 10821345 | 1.3021864 | 7.9277186 | 20-24 |
| AAGAG | 10842490 | 1.2961787 | 36.819817 | 7 |
| TCTGA | 10319860 | 1.2642772 | 8.118413 | 15-19 |
| CTCGT | 6427165 | 1.2615362 | 11.976622 | 40-44 |
| TCGTA | 9964240 | 1.2207105 | 7.954684 | 40-44 |
| TCTCG | 6086710 | 1.1947112 | 11.83411 | 40-44 |
| GTCTT | 9258435 | 1.1547358 | 7.420643 | 50-54 |
| TCATC | 9269615 | 1.1228186 | 6.6409526 | 35-39 |
| CTTGA | 9054800 | 1.1092957 | 6.7135553 | 60-64 |
| AGATC | 8427925 | 1.0141741 | 7.846209 | 35-39 |
| CATCT | 8359790 | 1.0126125 | 6.652397 | 35-39 |
| TATGC | 8129065 | 0.9958847 | 7.4326353 | 45-49 |
| CAGAT | 8267725 | 0.9948966 | 6.8818064 | 30-34 |
| GTATG | 7936395 | 0.98335946 | 7.3825326 | 45-49 |
| ATCTC | 7671680 | 0.92926246 | 6.4436584 | 40-44 |
| GATCA | 7467450 | 0.8985954 | 6.5926514 | 35-39 |
| CGTAT | 7312125 | 0.8958021 | 7.4932046 | 40-44 |