FastQCFastQC Report
Sun 20 Jan 2013
EL387_3KB_2.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename EL387_3KB_2.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34061927
Filtered Sequences 0
Sequence length 100
%GC 39

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGCCT 7114030 4.872587 5.361027 4
AGGCC 7087160 4.814541 5.615827 2
ACGTG 8349085 3.662855 4.782691 2
CACGT 8270920 3.6450915 5.047328 1
AAAAA 23815675 2.748152 5.290519 60-64
GGGGG 2156905 2.2566035 8.880421 40-44
GGAAG 3226835 1.3977273 6.6829305 5
ATCGG 2415900 1.0598875 7.594243 2
AGAGC 1998430 0.8695779 6.0785165 8
GAGCG 1246410 0.84288764 8.929587 9
CGGAA 1809505 0.7873708 6.5471125 4
GATCG 1496805 0.6566683 7.0495887 1
TCGGA 1438630 0.63114613 5.9716516 3
Produced by FastQC (version 0.10.0)