##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL387_3KB_2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34061927	
Filtered Sequences	0	
Sequence length	100	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16749043587581	34.0	31.0	34.0	30.0	34.0
2	32.293318490172325	34.0	31.0	34.0	31.0	34.0
3	32.373577748551924	34.0	31.0	34.0	31.0	34.0
4	35.744255396942165	37.0	37.0	37.0	35.0	37.0
5	35.75982442214735	37.0	37.0	37.0	35.0	37.0
6	35.78421593704901	37.0	37.0	37.0	35.0	37.0
7	35.74282447378858	37.0	37.0	37.0	35.0	37.0
8	35.76408152128328	37.0	37.0	37.0	35.0	37.0
9	37.45632271480119	39.0	38.0	39.0	35.0	39.0
10-14	37.738691178570136	39.4	38.2	39.4	35.2	39.4
15-19	38.867358925406656	41.0	39.0	41.0	36.0	41.0
20-24	38.74875827195566	41.0	39.0	41.0	35.4	41.0
25-29	38.59248166435211	41.0	39.0	41.0	35.0	41.0
30-34	38.34896876503787	40.2	38.4	41.0	34.2	41.0
35-39	38.368283438573506	40.6	38.6	41.0	34.4	41.0
40-44	38.20677113188576	40.4	38.4	41.0	34.2	41.0
45-49	38.090897898994385	40.0	38.0	41.0	33.8	41.0
50-54	37.369163429890506	39.6	37.2	40.6	32.8	41.0
55-59	37.22334618355562	39.8	36.8	41.0	32.0	41.0
60-64	36.70545809695382	39.0	35.6	41.0	31.2	41.0
65-69	36.034341932563	37.6	35.0	40.2	31.2	41.0
70-74	35.24811537526928	36.4	35.0	39.0	31.0	41.0
75-79	34.413673184139	35.2	35.0	37.2	30.4	39.4
80-84	33.69628540980667	35.0	34.0	36.2	30.0	38.0
85-89	33.16405496964397	35.0	34.0	35.6	29.6	36.6
90-94	32.73747602124801	35.0	34.0	35.0	29.0	36.0
95-99	32.31137708092675	35.0	34.0	35.0	29.0	36.0
100	31.19301770566298	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	263062.0
3	63234.0
4	25986.0
5	21084.0
6	28314.0
7	35185.0
8	26361.0
9	24178.0
10	26005.0
11	28088.0
12	29454.0
13	31993.0
14	36993.0
15	41337.0
16	43148.0
17	44677.0
18	46847.0
19	49684.0
20	52900.0
21	58934.0
22	67275.0
23	81243.0
24	99515.0
25	122969.0
26	152580.0
27	189852.0
28	235956.0
29	294848.0
30	373168.0
31	473646.0
32	612408.0
33	813171.0
34	1134201.0
35	1750672.0
36	3441260.0
37	8205898.0
38	1.1323048E7
39	3693275.0
40	19478.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.273431906243655	30.027004348569942	25.585511705065695	23.114052040120715
2	18.522237461821653	33.78531510006827	29.505849448089222	18.186597990020857
3	18.73037111038471	30.426298952697252	30.969782159877035	19.873547777041004
4	20.27037695195577	28.92826938417195	30.049392096929807	20.75196156694247
5	20.22616336415729	29.776770997131198	30.57623839074049	19.420827247971026
6	20.414996690433423	30.41901819695001	29.238539572499	19.927445540117567
7	20.100433113139673	31.094239914962174	29.341218423598292	19.464108548299865
8	18.574316127211475	31.184031367338672	30.84097385329961	19.400678652150244
9	19.71666118688129	30.211402868213323	30.613310555745947	19.458625389159444
10-14	19.716905713580786	30.547140238425314	30.283648594019326	19.452305453974578
15-19	19.695663950125812	30.118722569596784	30.65218996130256	19.533423518974843
20-24	20.00402081015285	30.193156311719232	30.313802342161257	19.489020535966663
25-29	19.781030273998336	30.509924751305746	30.167006382945438	19.542038591750476
30-34	20.035540303212347	30.212257687691245	30.2236944006025	19.5285076084939
35-39	19.43239310829733	30.384558798121002	30.479082367313875	19.703965726267796
40-44	20.170055980396565	30.171897053605036	30.118271919722815	19.539775046275583
45-49	19.69209575937953	30.241399123861758	30.24362770903207	19.822877407726647
50-54	19.591894345507175	30.433240031891636	30.32029112215873	19.654574500442465
55-59	19.43390141204263	30.70821939177425	30.513560193360746	19.344319002822374
60-64	19.280543050050863	31.09972717081505	30.227944929227025	19.39178484990706
65-69	19.34706981587709	31.002989097621743	30.311039631979085	19.338901454522077
70-74	19.39030806845202	30.801414302708086	30.351141725174102	19.457135903665794
75-79	19.45675664332041	30.69055033869221	30.412701466767057	19.439991551220327
80-84	19.47807329894948	30.605898927114687	30.393809156668645	19.52221861726719
85-89	19.566148617324686	30.53288570561947	30.424245444116693	19.47672023293915
90-94	19.590208913961593	30.543881841312846	30.34234906315446	19.5235601815711
95-99	19.700807652977286	30.48138561052096	30.334418121888625	19.483388614613137
100	19.871933402644903	30.458060858056417	30.158101839625928	19.51190389967275
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	44.38748394636437
2	36.70883545184251
3	38.60391888742571
4	41.02233851889824
5	39.64699061212831
6	40.342442230550986
7	39.56454166143954
8	37.97499477936172
9	39.17528657604073
10-14	39.16921116755536
15-19	39.22908746910066
20-24	39.49304134611951
25-29	39.323068865748816
30-34	39.564047911706254
35-39	39.13635883456513
40-44	39.70983102667215
45-49	39.51497316710618
50-54	39.246468845949636
55-59	38.778220414865004
60-64	38.67232789995793
65-69	38.685971270399165
70-74	38.847443972117816
75-79	38.896748194540734
80-84	39.00029191621667
85-89	39.04286885026384
90-94	39.11376909553269
95-99	39.18419626759042
100	39.38383730231765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	848.0
1	856.5
2	946.5
3	1110.0
4	1276.5
5	1442.0
6	1622.5
7	1827.0
8	2093.0
9	2364.0
10	2662.0
11	3145.0
12	3775.0
13	4617.5
14	5889.0
15	7666.5
16	10407.0
17	14969.0
18	22007.5
19	32701.5
20	49019.0
21	72335.5
22	104741.5
23	149034.0
24	207812.5
25	284755.5
26	374748.5
27	479412.0
28	606491.5
29	753734.0
30	915336.0
31	1086148.0
32	1262237.0
33	1441577.5
34	1605512.0
35	1718158.0
36	1759407.5
37	1728426.0
38	1646974.0
39	1532350.5
40	1400474.0
41	1271054.5
42	1156243.0
43	1065844.5
44	1014782.0
45	1017485.0
46	1068173.0
47	1141175.5
48	1198061.0
49	1197893.5
50	1119711.5
51	968527.5
52	771417.5
53	575280.5
54	409679.0
55	280717.0
56	186095.0
57	118950.5
58	72558.5
59	43346.5
60	25655.5
61	14767.5
62	9191.0
63	6102.5
64	4211.5
65	3491.5
66	3025.0
67	2637.0
68	2713.0
69	2456.0
70	1671.5
71	1357.5
72	1037.0
73	890.0
74	876.5
75	737.0
76	771.0
77	601.0
78	405.0
79	344.0
80	299.5
81	278.0
82	219.0
83	163.0
84	121.0
85	80.0
86	70.0
87	60.5
88	50.0
89	42.0
90	25.5
91	15.0
92	14.5
93	14.5
94	12.5
95	6.0
96	6.0
97	8.0
98	6.5
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026108329103048104
2	2.3780216545000522E-4
3	0.001541310331620404
4	0.0
5	0.0
6	1.9082889820062147E-4
7	0.0024954548226235117
8	0.0
9	0.001098000121954345
10-14	0.004157721317411079
15-19	0.012698048469189663
20-24	0.0033163126678064925
25-29	0.00856498811708451
30-34	0.004230529881647624
35-39	0.019098743297758815
40-44	0.03588111735428239
45-49	0.03939589207621753
50-54	0.027487581662658134
55-59	0.0357049676020972
60-64	0.03193536290533416
65-69	0.01082851243266419
70-74	0.0037273287562386006
75-79	0.001986382038808315
80-84	0.0023016900952198037
85-89	0.0020715210856978236
90-94	0.003711475278541933
95-99	0.002314607743713384
100	8.044172016456966E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.4061927E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	38.64496756114212
#Duplication Level	Relative count
1	100.0
2	24.8480793022152
3	6.244115747621011
4	2.39493934843333
5	1.5141494386131449
6	1.0893161428871547
7	0.8800118268609799
8	0.7905322865523923
9	0.7150582395094965
10++	17.140389508329378
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGCCT	7114030	4.872587	5.361027	4
AGGCC	7087160	4.814541	5.615827	2
ACGTG	8349085	3.662855	4.782691	2
CACGT	8270920	3.6450915	5.047328	1
AAAAA	23815675	2.748152	5.290519	60-64
GGGGG	2156905	2.2566035	8.880421	40-44
GGAAG	3226835	1.3977273	6.6829305	5
ATCGG	2415900	1.0598875	7.594243	2
AGAGC	1998430	0.8695779	6.0785165	8
GAGCG	1246410	0.84288764	8.929587	9
CGGAA	1809505	0.7873708	6.5471125	4
GATCG	1496805	0.6566683	7.0495887	1
TCGGA	1438630	0.63114613	5.9716516	3
>>END_MODULE