##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL387_3KB_1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34061927	
Filtered Sequences	0	
Sequence length	100	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62847539424296	34.0	31.0	34.0	31.0	34.0
2	32.89108514030929	34.0	31.0	34.0	31.0	34.0
3	32.97418046841566	34.0	31.0	34.0	31.0	34.0
4	36.19844831444797	37.0	37.0	37.0	35.0	37.0
5	36.23857076553537	37.0	37.0	37.0	35.0	37.0
6	36.267646689513484	37.0	37.0	37.0	35.0	37.0
7	36.268734590382984	37.0	37.0	37.0	35.0	37.0
8	36.285011032992934	37.0	37.0	37.0	35.0	37.0
9	38.0845050839314	39.0	39.0	39.0	35.0	39.0
10-14	38.35785792741556	39.4	39.0	39.4	35.4	39.4
15-19	39.4569446937045	41.0	39.0	41.0	36.6	41.0
20-24	39.21626081812693	41.0	39.0	41.0	36.0	41.0
25-29	38.01375450073626	40.8	38.2	41.0	29.2	41.0
30-34	36.44843398906938	40.0	36.0	41.0	23.4	41.0
35-39	37.42986509835454	40.0	36.6	41.0	29.6	41.0
40-44	36.27751668894129	40.0	36.0	41.0	23.0	41.0
45-49	38.45797518736976	40.0	38.0	41.0	34.2	41.0
50-54	38.27186213510468	40.0	37.8	41.0	34.0	41.0
55-59	37.71217991278063	39.6	36.8	41.0	33.0	41.0
60-64	36.93096293700588	38.8	35.4	40.6	31.6	41.0
65-69	36.36846896536417	37.2	35.0	39.8	32.0	41.0
70-74	35.19952786581922	36.2	34.8	38.8	31.0	40.8
75-79	34.127355660177415	35.0	33.8	37.0	29.8	39.2
80-84	33.900144510320864	35.0	34.0	36.0	31.0	37.6
85-89	33.354726566115886	35.0	34.0	35.4	29.8	36.4
90-94	32.946962472205406	35.0	34.0	35.0	29.6	36.0
95-99	32.587030123104896	35.0	34.0	35.0	29.0	36.0
100	31.445081395424282	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	543.0
3	85.0
4	207.0
5	406.0
6	848.0
7	2230.0
8	5735.0
9	10516.0
10	13312.0
11	15494.0
12	18746.0
13	23666.0
14	29644.0
15	34840.0
16	39285.0
17	43073.0
18	47173.0
19	52195.0
20	55702.0
21	62048.0
22	73537.0
23	91277.0
24	119525.0
25	154342.0
26	197840.0
27	273115.0
28	314522.0
29	361443.0
30	461361.0
31	599463.0
32	806842.0
33	1187661.0
34	2025110.0
35	3367595.0
36	3978374.0
37	7011719.0
38	9554068.0
39	3014342.0
40	14043.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.35040445840127	30.10625315696171	25.393766971194403	22.14957541344261
2	18.14411479973222	32.531611223044784	30.898809735964868	18.42546424125813
3	18.394445540000422	29.233148296424154	32.64194405395385	19.730462109621577
4	20.056211791029867	29.011142392077023	30.66547173135493	20.267174085538183
5	20.38950409352941	29.96995443035269	30.721236059251726	18.91930541686617
6	20.26038045351926	30.61328268362503	29.6208725947889	19.50546426806681
7	19.464512386512954	31.645581883843505	29.72533526949312	19.164570460150422
8	18.62040285480258	31.17047199363512	30.836932173484154	19.372192978078147
9	20.10238880495516	29.989909261446073	30.537144889072188	19.370557044526578
10-14	19.524841286123472	30.639337930529116	30.225002557107462	19.61081822623995
15-19	19.525572036579238	30.30028188603594	30.534137748318567	19.64000832906625
20-24	19.632443744750734	30.28637872334029	30.383031615863455	19.698145916045522
25-29	19.967051217315678	30.06881380953443	30.135485342423117	19.828649630726776
30-34	19.778313337159616	30.241822672043934	30.139924296167646	19.839939694628796
35-39	19.63901065397836	30.51861337406679	30.250779239094555	19.591596732860296
40-44	19.820115471567938	30.18557291609445	30.220652407334665	19.773659205002946
45-49	19.91377453677542	30.37258142824053	30.386499842060676	19.327144192923367
50-54	19.57276137165412	30.244696254797553	30.26487641436289	19.917665959185438
55-59	19.601118256666314	30.300359148456558	30.619016445342467	19.479506149534664
60-64	19.664879589188978	30.320015233493365	30.493856759062542	19.521248418255116
65-69	19.402330686662687	31.08785777521469	30.241433929249684	19.26837760887294
70-74	19.562564742786964	30.93632746343812	30.15934882407781	19.341758969697107
75-79	19.52003274105663	30.729329176258986	30.348734359842666	19.401903722841716
80-84	19.51523026689165	30.61413549771271	30.360687295750765	19.509946939644877
85-89	19.599395744044052	30.526063611696795	30.378558186970935	19.495982457288218
90-94	19.595105100483256	30.537885109953795	30.312463172263598	19.55454661729935
95-99	19.657910192329712	30.526140901926873	30.286105889605658	19.529843016137754
100	19.721180559735092	30.555683592046968	30.098378020678496	19.624757827539447
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	44.499979871843884
2	36.569579040990355
3	38.124907649621996
4	40.32338587656805
5	39.308809510395584
6	39.76584472158607
7	38.62908284666337
8	37.992595832880724
9	39.47294584948174
10-14	39.135659512363425
15-19	39.16558036564549
20-24	39.33058966079626
25-29	39.79570084804245
30-34	39.61825303178841
35-39	39.230607386838656
40-44	39.59377467657089
45-49	39.24091872969879
50-54	39.49042733083956
55-59	39.08062440620098
60-64	39.18612800744409
65-69	38.67070829553562
70-74	38.90432371248407
75-79	38.92193646389835
80-84	39.025177206536526
85-89	39.095378201332274
90-94	39.14965171778261
95-99	39.18775320846747
100	39.345938387274536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	77.0
2	42.0
3	46.0
4	66.0
5	70.5
6	74.5
7	109.5
8	161.0
9	250.0
10	441.5
11	716.0
12	1100.0
13	1813.5
14	2940.5
15	4591.5
16	7372.5
17	11802.0
18	18779.5
19	29823.5
20	46475.5
21	70014.0
22	102370.5
23	146876.0
24	206783.0
25	283651.5
26	374922.5
27	482996.5
28	611862.0
29	761086.0
30	926461.0
31	1101346.0
32	1282981.5
33	1465916.0
34	1627716.5
35	1735223.0
36	1773340.0
37	1745021.0
38	1661949.5
39	1547264.5
40	1420931.5
41	1296131.5
42	1183843.0
43	1095638.0
44	1046694.0
45	1046116.0
46	1090110.0
47	1151991.5
48	1190568.0
49	1173473.0
50	1084109.0
51	925104.0
52	727159.0
53	535086.0
54	375829.5
55	253785.5
56	165041.0
57	103831.5
58	63142.0
59	36638.5
60	20257.5
61	11199.0
62	6791.5
63	4778.0
64	3578.5
65	2516.0
66	2009.5
67	1804.0
68	1443.5
69	1276.0
70	1331.0
71	1068.5
72	714.5
73	655.5
74	646.5
75	414.5
76	434.5
77	497.5
78	227.0
79	161.5
80	127.0
81	60.5
82	50.5
83	19.5
84	14.5
85	10.0
86	8.5
87	7.0
88	7.0
89	4.0
90	1.5
91	1.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08806606860498527
2	0.0031736313685364896
3	2.1725136102839984E-4
4	0.0011449733892037288
5	0.0
6	0.0
7	0.0
8	7.926738848333507E-5
9	0.0
10-14	8.807487609259453E-6
15-19	1.6440643537284312E-5
20-24	1.1449733892037287E-4
25-29	2.392858748126611
30-34	2.4787851844083866
35-39	0.0018278472618416452
40-44	2.1973066879040637
45-49	0.005905126859088155
50-54	0.028386532564643213
55-59	0.04110513183825448
60-64	0.015829991063042323
65-69	0.008765798834575626
70-74	0.031247204540130684
75-79	0.016452386854096657
80-84	0.01128415312498321
85-89	0.009533811754103049
90-94	0.018826885513553004
95-99	0.006299702303982978
100	0.0013416739458105233
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3.4061927E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	37.08636511921478
#Duplication Level	Relative count
1	100.0
2	22.23675113475817
3	5.348393302672318
4	2.2289355818077974
5	1.3737337301229446
6	1.057354134728109
7	0.9175759761926233
8	0.7665253855171792
9	0.7695313674211681
10++	15.60029458622659
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC	209623	0.615417325038598	TruSeq Adapter, Index 8 (97% over 36bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGGCC	7020635	4.849875	5.5223064	7
GGCCT	6939455	4.8217864	5.455381	8
CACGT	8523095	3.818797	6.1559443	10-14
ACGTG	8185840	3.6546605	4.44241	9
GGAAG	3283190	1.4521313	13.515304	5
GAAGA	4744515	1.353157	9.191351	6
ATCGG	2489540	1.1114832	13.440582	2
AGAGC	2149870	0.9542616	13.069224	8
AAGAG	3127015	0.8918388	8.655189	7
GAGCA	1946865	0.864154	13.042246	9
CGGAA	1932830	0.8579242	12.961918	4
GATCG	1621895	0.7241133	12.890784	1
TCGGA	1584615	0.7074692	12.866017	3
>>END_MODULE