EL387_3KB_1.fastq FastQC Report

Summary

Basic Statistics
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Kmer Content

Basic Statistics

Measure	Value
Filename	EL387_3KB_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34061927
Filtered Sequences	0
Sequence length	100
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per base sequence content

Per base GC content

Per base GC content graph

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC	209623	0.615417325038598	TruSeq Adapter, Index 8 (97% over 36bp)

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGGCC	7020635	4.849875	5.5223064	7
GGCCT	6939455	4.8217864	5.455381	8
CACGT	8523095	3.818797	6.1559443	10-14
ACGTG	8185840	3.6546605	4.44241	9
GGAAG	3283190	1.4521313	13.515304	5
GAAGA	4744515	1.353157	9.191351	6
ATCGG	2489540	1.1114832	13.440582	2
AGAGC	2149870	0.9542616	13.069224	8
AAGAG	3127015	0.8918388	8.655189	7
GAGCA	1946865	0.864154	13.042246	9
CGGAA	1932830	0.8579242	12.961918	4
GATCG	1621895	0.7241133	12.890784	1
TCGGA	1584615	0.7074692	12.866017	3