##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL387_2KB_2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25751908	
Filtered Sequences	0	
Sequence length	100	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673419499634747	33.0	31.0	34.0	30.0	34.0
2	31.773291905205625	34.0	31.0	34.0	30.0	34.0
3	31.812495951756272	34.0	31.0	34.0	30.0	34.0
4	35.1569273235987	37.0	35.0	37.0	33.0	37.0
5	35.18834732556516	37.0	35.0	37.0	33.0	37.0
6	35.254151187554726	37.0	36.0	37.0	33.0	37.0
7	35.0907804578985	37.0	36.0	37.0	33.0	37.0
8	35.17402640612105	37.0	36.0	37.0	33.0	37.0
9	36.697557788727735	39.0	38.0	39.0	33.0	39.0
10-14	36.97609269185025	39.4	38.2	39.4	33.4	39.4
15-19	38.05051052527836	41.0	39.0	41.0	33.8	41.0
20-24	37.94359674630711	41.0	39.0	41.0	33.4	41.0
25-29	37.74792032497165	40.4	38.0	41.0	33.2	41.0
30-34	37.43604828815014	40.0	38.0	41.0	32.2	41.0
35-39	37.45535186751987	40.0	38.0	41.0	32.4	41.0
40-44	37.281065635990934	40.0	38.0	41.0	31.6	41.0
45-49	37.10858407850789	40.0	38.0	41.0	31.0	41.0
50-54	36.35243082570814	39.4	37.0	40.6	30.2	41.0
55-59	36.1817537869427	39.2	35.8	41.0	29.2	41.0
60-64	35.6379885404996	38.6	35.0	40.6	29.0	41.0
65-69	34.87242921184714	37.0	35.0	39.6	28.8	41.0
70-74	34.08070475399337	35.8	34.6	38.6	28.2	40.8
75-79	33.2853097409326	35.0	34.0	36.8	27.6	39.2
80-84	32.61408597762931	35.0	34.0	36.0	26.6	37.6
85-89	32.11177280533932	35.0	34.0	35.0	25.8	36.4
90-94	31.68579459820997	35.0	33.4	35.0	25.0	36.0
95-99	31.234880739710626	35.0	33.0	35.0	22.8	35.8
100	30.063100450653987	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	515513.0
3	128623.0
4	36597.0
5	20986.0
6	41598.0
7	59510.0
8	34631.0
9	26566.0
10	28320.0
11	30038.0
12	31037.0
13	34741.0
14	45614.0
15	52815.0
16	53257.0
17	52594.0
18	54195.0
19	54747.0
20	53807.0
21	55501.0
22	60280.0
23	70943.0
24	85532.0
25	104396.0
26	126788.0
27	157475.0
28	191830.0
29	239098.0
30	299779.0
31	379183.0
32	488191.0
33	645795.0
34	902572.0
35	1399445.0
36	2771509.0
37	6386446.0
38	7772971.0
39	2245983.0
40	13002.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.08178146407794	30.81300366482483	27.298781105488164	19.806433765609064
2	19.11493309163171	33.79026859456876	28.714829580666034	18.379968733133502
3	19.406845219175853	30.373449055823603	31.397327634349402	18.82237809065114
4	19.939753590297077	29.354298718370693	29.587691133410388	21.118256557921843
5	21.569854163815748	29.839121046875437	29.46521088845145	19.125813900857366
6	22.4002201316464	29.740648278534255	28.18319202769912	19.675939562120224
7	20.7083074802435	31.427874209252714	28.67106303167758	19.192755278826205
8	18.941714920696363	32.67497305442377	29.078105591243958	19.305206433635906
9	21.270542412898617	30.377642554249057	29.056784821137327	19.295030211715
10-14	20.843129491967733	30.18190601660335	29.42793327980754	19.547031211621373
15-19	21.053513872891838	29.259924852814724	29.77238163261953	19.914179641673904
20-24	21.745750381404182	29.711688487659092	29.146785227833576	19.39577590310315
25-29	20.746576539711008	30.978236643919395	28.77891129898693	19.49627551738267
30-34	21.61416360919441	29.629763936895394	29.253602308564297	19.502470145345896
35-39	19.77793561578148	29.92018502576685	29.952641530124353	20.349237828327315
40-44	22.18645350334754	29.03527835052219	29.04142139983553	19.736846746294738
45-49	20.87221184974899	29.065618574228747	29.07713755913347	20.985032016888795
50-54	20.413520508341655	29.498948334989972	29.68360012414114	20.403931032527233
55-59	19.71625908765424	30.49153075468357	30.195618534162485	19.596591623499705
60-64	19.493199122592536	32.13457876796342	28.890161223508997	19.48206088593505
65-69	19.675647533050512	31.75985816074931	29.045314856813327	19.51917944938685
70-74	19.90687847062808	30.951533809882438	29.344875867435427	19.796711852054056
75-79	20.1052930068412	30.52060228859339	29.476357567881763	19.89774713668365
80-84	20.261301297748105	30.268204941117954	29.4886421051756	19.98185165595834
85-89	20.449536897073312	30.107655740034634	29.498631320016344	19.94417604287571
90-94	20.60613486030563	30.01050366894461	29.42219957110795	19.96116189964181
95-99	20.811683720325664	29.921130024366573	29.381778014737996	19.885408240569767
100	21.058155174387547	29.976229940954635	29.05704487979135	19.908570004866473
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.888215229687006
2	37.49490182476521
3	38.22922330982699
4	41.05801014821892
5	40.695668064673114
6	42.07615969376663
7	39.9010627590697
8	38.24692135433227
9	40.565572624613615
10-14	40.3901607035891
15-19	40.967693514565745
20-24	41.14152628450733
25-29	40.24285205709368
30-34	41.1166337545403
35-39	40.1271734441088
40-44	41.923300249642274
45-49	41.85724386663778
50-54	40.81745154086889
55-59	39.31285071115395
60-64	38.975260008527584
65-69	39.19482698243736
70-74	39.70359032268214
75-79	40.00304014352485
80-84	40.24315295370645
85-89	40.393712939949026
90-94	40.56729675994744
95-99	40.697091960895435
100	40.96672517925401
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	362.0
1	282.0
2	230.5
3	298.5
4	375.5
5	454.5
6	539.5
7	617.5
8	754.0
9	933.5
10	1198.0
11	1600.5
12	2023.0
13	2739.5
14	3816.0
15	5249.0
16	7543.0
17	11285.5
18	16516.0
19	24496.5
20	36373.5
21	53165.0
22	76147.0
23	107207.0
24	147252.0
25	199403.5
26	260727.5
27	329946.0
28	411731.5
29	508208.5
30	614096.0
31	718420.0
32	826358.0
33	938644.5
34	1033718.0
35	1097316.5
36	1125048.0
37	1113041.5
38	1072157.5
39	1016465.5
40	953662.0
41	892923.0
42	841661.5
43	808727.5
44	810307.5
45	855278.0
46	933295.5
47	1028985.5
48	1108774.5
49	1134519.0
50	1084315.5
51	950025.5
52	763216.5
53	576788.5
54	417832.0
55	291277.5
56	196999.5
57	129198.5
58	81848.0
59	48809.5
60	27574.5
61	15722.0
62	9365.5
63	5980.5
64	4065.5
65	2894.5
66	2109.0
67	1669.0
68	1329.0
69	1056.0
70	885.0
71	750.0
72	619.5
73	513.0
74	423.0
75	341.5
76	299.0
77	246.5
78	192.5
79	159.0
80	140.0
81	109.5
82	88.5
83	79.5
84	59.0
85	43.5
86	42.5
87	32.5
88	24.0
89	20.0
90	17.5
91	13.0
92	9.5
93	5.5
94	5.0
95	5.5
96	4.0
97	4.0
98	2.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025675767403331825
2	1.8251074832979368E-4
3	0.0016192974905005096
4	0.0
5	0.0
6	1.7474433350724925E-4
7	0.002461953498746578
8	0.0
9	0.0011960278826718394
10-14	0.004205513626407799
15-19	0.012577708805110673
20-24	0.0034148925974727775
25-29	0.008376078386114148
30-34	0.004332106188015272
35-39	0.018773754550536606
40-44	0.034919354325124184
45-49	0.03905108701071781
50-54	0.027252349612308338
55-59	0.03522535106913243
60-64	0.03167221628781836
65-69	0.010713769247700015
70-74	0.0035469216494560325
75-79	0.0019113146878281795
80-84	0.0022437172422330805
85-89	0.002045673664258198
90-94	0.0035181859146126183
95-99	0.0021839158480994885
100	7.145101636740858E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5751908E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	89.49988515499388
#Duplication Level	Relative count
1	100.0
2	100.59375
3	128.37053571428572
4	121.64285714285714
5	99.17410714285714
6	71.85714285714286
7	51.25892857142857
8	37.191964285714285
9	27.165178571428573
10++	155.60267857142858
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG	32084	0.12458882658325744	Illumina Single End PCR Primer 1 (98% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGCCT	6266435	5.196641	5.859822	4
AGGCC	6368690	5.12483	5.919339	7
CACGT	7403740	4.175181	4.73397	8
ACGTG	7330425	3.9816277	4.676036	2
GGGGG	3499885	3.8421648	23.744684	40-44
AAAAA	21566940	3.442868	12.9381275	60-64
GCCTT	5746290	3.3395236	3.848077	5
AAGGC	5921510	3.120981	3.6350923	1
GCGCC	2427400	2.9822316	8.251737	45-49
GGCGC	2426995	2.871946	7.686119	40-44
GGAAA	5197490	1.7942446	5.2448072	6
GGAAG	3523795	1.7888614	17.801622	5
GGGAA	2994300	1.5200622	7.908709	20-24
ATCGG	2796170	1.5187807	22.008387	2
GGGCG	1254535	1.4298733	6.3584743	40-44
GAAGA	4068350	1.40445	12.476545	6
CGCCG	1133855	1.3930206	8.575072	45-49
GGCGG	1168985	1.3323667	5.289439	10-14
GGGGA	1712810	1.2786548	7.2649198	20-24
CGGGG	1113405	1.2690187	6.2270393	35-39
GGGGC	1090790	1.2432427	7.1793885	40-44
AGGGG	1623930	1.2123036	5.469653	25-29
GAGCG	1536280	1.1907129	24.848324	9
AGAGG	2328015	1.1818213	5.727788	8
AAGAG	3372580	1.1642607	11.690672	7
AGAGC	2142615	1.1292831	17.321936	8
CGGAA	2118660	1.1166575	18.959496	4
GATCG	2010015	1.0917691	21.519466	1
GAGGG	1408420	1.0514201	7.551676	9
CGGGA	1333015	1.03317	9.485013	4
GGGAG	1366255	1.0199429	7.545621	5
TCGGA	1810890	0.98361146	17.869192	3
TCGGG	1151695	0.91991365	7.091132	3
GAGAG	1763750	0.895371	5.7175546	7
GGAGA	1549645	0.7866803	6.1333914	6
CTCGG	945650	0.7842104	5.736291	35-39
>>END_MODULE