FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | EL387_2KB_2.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 25751908 |
| Filtered Sequences | 0 |
| Sequence length | 100 |
| %GC | 40 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
![[FAIL]](Icons/error.png)
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG | 32084 | 0.12458882658325744 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| GGCCT | 6266435 | 5.196641 | 5.859822 | 4 |
| AGGCC | 6368690 | 5.12483 | 5.919339 | 7 |
| CACGT | 7403740 | 4.175181 | 4.73397 | 8 |
| ACGTG | 7330425 | 3.9816277 | 4.676036 | 2 |
| GGGGG | 3499885 | 3.8421648 | 23.744684 | 40-44 |
| AAAAA | 21566940 | 3.442868 | 12.9381275 | 60-64 |
| GCCTT | 5746290 | 3.3395236 | 3.848077 | 5 |
| AAGGC | 5921510 | 3.120981 | 3.6350923 | 1 |
| GCGCC | 2427400 | 2.9822316 | 8.251737 | 45-49 |
| GGCGC | 2426995 | 2.871946 | 7.686119 | 40-44 |
| GGAAA | 5197490 | 1.7942446 | 5.2448072 | 6 |
| GGAAG | 3523795 | 1.7888614 | 17.801622 | 5 |
| GGGAA | 2994300 | 1.5200622 | 7.908709 | 20-24 |
| ATCGG | 2796170 | 1.5187807 | 22.008387 | 2 |
| GGGCG | 1254535 | 1.4298733 | 6.3584743 | 40-44 |
| GAAGA | 4068350 | 1.40445 | 12.476545 | 6 |
| CGCCG | 1133855 | 1.3930206 | 8.575072 | 45-49 |
| GGCGG | 1168985 | 1.3323667 | 5.289439 | 10-14 |
| GGGGA | 1712810 | 1.2786548 | 7.2649198 | 20-24 |
| CGGGG | 1113405 | 1.2690187 | 6.2270393 | 35-39 |
| GGGGC | 1090790 | 1.2432427 | 7.1793885 | 40-44 |
| AGGGG | 1623930 | 1.2123036 | 5.469653 | 25-29 |
| GAGCG | 1536280 | 1.1907129 | 24.848324 | 9 |
| AGAGG | 2328015 | 1.1818213 | 5.727788 | 8 |
| AAGAG | 3372580 | 1.1642607 | 11.690672 | 7 |
| AGAGC | 2142615 | 1.1292831 | 17.321936 | 8 |
| CGGAA | 2118660 | 1.1166575 | 18.959496 | 4 |
| GATCG | 2010015 | 1.0917691 | 21.519466 | 1 |
| GAGGG | 1408420 | 1.0514201 | 7.551676 | 9 |
| CGGGA | 1333015 | 1.03317 | 9.485013 | 4 |
| GGGAG | 1366255 | 1.0199429 | 7.545621 | 5 |
| TCGGA | 1810890 | 0.98361146 | 17.869192 | 3 |
| TCGGG | 1151695 | 0.91991365 | 7.091132 | 3 |
| GAGAG | 1763750 | 0.895371 | 5.7175546 | 7 |
| GGAGA | 1549645 | 0.7866803 | 6.1333914 | 6 |
| CTCGG | 945650 | 0.7842104 | 5.736291 | 35-39 |