##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	EL387_2KB_1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25751908	
Filtered Sequences	0	
Sequence length	100	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33787581875487	34.0	31.0	34.0	31.0	34.0
2	32.63359604266993	34.0	31.0	34.0	31.0	34.0
3	32.71841830127694	34.0	31.0	34.0	31.0	34.0
4	35.95773454922253	37.0	37.0	37.0	35.0	37.0
5	36.017960805078985	37.0	37.0	37.0	35.0	37.0
6	36.059646298829584	37.0	37.0	37.0	35.0	37.0
7	36.07671668444917	37.0	37.0	37.0	35.0	37.0
8	36.126531090434156	37.0	37.0	37.0	35.0	37.0
9	37.90530138582353	39.0	38.0	39.0	35.0	39.0
10-14	38.18150660525814	39.4	38.2	39.4	35.2	39.4
15-19	39.251649081691355	41.0	39.0	41.0	36.0	41.0
20-24	39.00323264590725	41.0	39.0	41.0	35.4	41.0
25-29	37.77864072829089	40.8	38.2	41.0	28.8	41.0
30-34	36.09911690426977	40.0	35.4	41.0	23.0	41.0
35-39	37.084414250004315	40.0	36.2	41.0	29.2	41.0
40-44	35.804078866699896	40.0	35.0	41.0	21.8	41.0
45-49	38.01848121700342	40.0	38.0	41.0	33.2	41.0
50-54	37.775486297947324	40.0	37.2	41.0	32.6	41.0
55-59	37.16408940261825	39.2	36.2	41.0	31.4	41.0
60-64	36.272142631140184	38.2	35.0	40.2	30.4	41.0
65-69	35.58405195451925	36.8	35.0	39.4	30.8	41.0
70-74	33.97752547112238	35.6	34.2	38.2	28.2	40.2
75-79	32.8720208692886	35.0	33.4	36.2	26.4	39.0
80-84	32.69350959159997	35.0	34.0	35.8	27.6	37.2
85-89	32.17339604506198	35.0	33.8	35.0	26.6	36.2
90-94	31.755204460966546	35.0	33.0	35.0	25.2	36.0
95-99	31.358017673874883	35.0	33.0	35.0	24.2	35.6
100	30.174833802606006	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9745.0
3	1110.0
4	3241.0
5	5342.0
6	8440.0
7	11945.0
8	15768.0
9	20240.0
10	22471.0
11	25173.0
12	29472.0
13	34287.0
14	39351.0
15	44249.0
16	48662.0
17	53748.0
18	60133.0
19	67389.0
20	74308.0
21	85080.0
22	101827.0
23	128039.0
24	171201.0
25	204749.0
26	258501.0
27	358355.0
28	295474.0
29	304911.0
30	381078.0
31	486904.0
32	645446.0
33	938916.0
34	1571932.0
35	2521325.0
36	2983987.0
37	5363604.0
38	6533069.0
39	1832018.0
40	10418.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.667036140485813	30.41110504605759	27.265127455836573	19.656731357620018
2	18.121589227346526	33.15391208075141	29.89616766332196	18.828331028580106
3	18.77949400439052	28.95281984466821	33.534002458231726	18.73368369270954
4	19.587889524491498	29.04666857463947	29.565044333184865	21.800397567684172
5	22.197485328077438	29.651496114385	29.390125189947092	18.760893367590473
6	22.242747993663226	29.612772770079793	28.62837192490747	19.51610731134951
7	19.119705615599432	32.77048442391142	28.88292393713118	19.22688602335796
8	18.667866733551058	32.66603882944861	29.16027781719235	19.505816619807984
9	22.23164202046699	29.254022653389413	29.059679772077473	19.45465555406613
10-14	19.650648439033503	30.540559167344533	29.285078427054117	20.523713966567847
15-19	19.844901251066357	29.8035919162387	29.700790194793914	20.650716637901027
20-24	19.949520725787284	30.276912876381612	29.62250384882642	20.151062549004685
25-29	20.292780891373717	29.577546344779034	29.272811829145663	20.85686093470159
30-34	19.70223750251815	30.047991315176215	29.40010306571037	20.849668116595264
35-39	20.06059926085892	29.68372457466706	29.383584813739393	20.872091350734628
40-44	19.789263618121808	29.398162135186496	29.96253981612459	20.850034430567106
45-49	20.854257130546443	29.610209770209305	30.00040216812268	19.53513093112157
50-54	20.065391494382425	28.96486964626474	29.47310563856852	21.496633220784318
55-59	20.134622532161682	29.070427628540386	30.621479510712625	20.173470328585307
60-64	20.197439395983803	29.534633869686	30.073933046640978	20.19399368768922
65-69	19.520077860990703	32.12957104423164	28.83460027697737	19.51575081780029
70-74	19.773849146027658	31.57853537527826	28.951099563596262	19.696515915097816
75-79	19.886190995400284	30.76229596932844	29.334085637717976	20.0174273975533
80-84	20.022279180845953	30.31968667937786	29.431474893050087	20.226559246726104
85-89	20.163125321967375	30.101610659424296	29.40219444699811	20.33306957161022
90-94	20.238144702714116	30.006248077697546	29.335863169778335	20.419744049810003
95-99	20.318781333696183	29.94988410527989	29.25548897515325	20.47584558587068
100	20.39554657695731	30.018794555229118	28.94787498116137	20.6377838866522
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.32376749810583
2	36.949920255926635
3	37.51317769710006
4	41.388287092175666
5	40.95837869566791
6	41.75885530501274
7	38.34659163895739
8	38.17368335335904
9	41.686297574533114
10-14	40.17436240560135
15-19	40.49561788896738
20-24	40.100583274791965
25-29	41.1496418260753
30-34	40.55190561911341
35-39	40.93269061159355
40-44	40.639298048688914
45-49	40.389388061668015
50-54	41.56202471516674
55-59	40.30809286074699
60-64	40.39143308367302
65-69	39.035828678791
70-74	39.47036506112548
75-79	39.90361839295358
80-84	40.24883842757205
85-89	40.496194893577595
90-94	40.657888752524116
95-99	40.79462691956686
100	41.033330463609516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	158.5
2	66.0
3	58.0
4	67.0
5	102.0
6	131.0
7	150.0
8	199.0
9	310.5
10	485.0
11	793.0
12	1168.0
13	1802.5
14	2806.5
15	4208.5
16	6519.0
17	10358.0
18	16078.0
19	24091.0
20	35965.5
21	52993.5
22	76659.5
23	108705.0
24	149733.0
25	201138.0
26	261114.0
27	332341.0
28	416705.0
29	514171.0
30	618504.5
31	723693.5
32	833882.5
33	945790.5
34	1040263.0
35	1100531.5
36	1126166.5
37	1118336.0
38	1081216.5
39	1029996.0
40	975968.0
41	925440.0
42	886182.5
43	861889.0
44	866062.5
45	905000.5
46	971929.0
47	1052931.5
48	1108946.5
49	1110339.5
50	1042189.0
51	896652.5
52	709984.0
53	527623.5
54	373309.0
55	256551.0
56	171359.0
57	109950.0
58	67267.0
59	38619.5
60	21180.0
61	11752.0
62	6786.0
63	4216.5
64	2815.5
65	1931.0
66	1449.0
67	1180.5
68	934.5
69	733.5
70	599.5
71	509.0
72	443.5
73	374.0
74	291.5
75	232.0
76	184.5
77	133.0
78	98.5
79	77.0
80	54.0
81	42.0
82	35.0
83	24.5
84	16.0
85	11.5
86	7.5
87	5.5
88	5.5
89	3.5
90	2.0
91	2.0
92	1.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0839665938539389
2	0.0030211353659697756
3	2.4852527432142114E-4
4	0.0012348599567845613
5	0.0
6	0.0
7	0.0
8	3.883207411272205E-5
9	0.0
10-14	1.397954668057994E-5
15-19	1.7862754091852143E-5
20-24	1.2503927864296503E-4
25-29	2.384648935527418
30-34	2.4708087649272437
35-39	0.0019602431012102095
40-44	2.1871917218716375
45-49	0.005759573232398936
50-54	0.028014234906399944
55-59	0.04047156428176118
60-64	0.015285081012249657
65-69	0.008631593433775859
70-74	0.03104468997015677
75-79	0.01620773109316793
80-84	0.011053161575445206
85-89	0.0093383371826274
90-94	0.01859668029258259
95-99	0.006036834241563771
100	0.0011688454307929338
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2.5751908E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	90.73280925273335
#Duplication Level	Relative count
1	100.0
2	144.69578783151326
3	164.75721528861155
4	139.69871294851794
5	102.41809672386896
6	70.11505460218409
7	47.80616224648986
8	33.487714508580346
9	22.474648985959437
10++	149.58560842433698
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC	562799	2.185465247856586	TruSeq Adapter, Index 1 (100% over 50bp)
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGCCT	6190110	5.2135425	6.1934404	8
AGGCC	6152870	5.0764184	6.2236757	7
CACGT	8388365	4.7045536	12.692248	10-14
ACGTG	7130140	4.0457473	4.8640165	8
GCCTT	5707945	3.267952	3.671505	9
AAAAA	19269690	3.1996317	12.55412	65-69
AAGGC	5687595	3.161364	3.7519438	6
ACACG	3857810	2.1194692	9.933431	10-14
ACGTC	3736085	2.095356	9.959069	15-19
GGAAG	3599105	2.023951	44.398544	5
GAAAA	7957320	1.9842001	5.4188795	60-64
TCACG	3401530	1.9077233	10.521081	35-39
GCACA	3411350	1.8741856	9.645272	10-14
CAGTC	3241835	1.8181596	9.5395155	25-29
ATCGG	3174425	1.801216	44.334946	2
CCGTC	2100825	1.7488991	13.344373	50-54
GCCGT	2073290	1.7462022	13.576636	45-49
CGTCT	3004390	1.7200941	9.26222	15-19
TGAAA	6746140	1.7172314	5.1828566	60-64
TGCCG	1947055	1.6398823	13.767243	45-49
GTCTG	2817745	1.6321391	9.744313	15-19
GAAGA	4273985	1.6004608	29.929108	6
ATCAC	4065300	1.5182394	6.6124535	30-34
AGCAC	2725390	1.497321	9.220668	10-14
CTTCT	3772115	1.4680548	6.963211	50-54
CACAC	2671245	1.4505756	9.109162	10-14
AGAGC	2589870	1.4395403	43.458027	8
TCTTC	3573310	1.3906825	6.845307	50-54
CGGAA	2498895	1.3889732	43.030445	4
TCCAG	2472550	1.3867116	9.139956	25-29
CCAGT	2456855	1.3779092	9.152795	25-29
CTGCT	2371950	1.3580052	9.450719	55-59
CTCCA	2447090	1.3565363	9.164733	20-24
GATCG	2380180	1.3505495	43.663857	1
TGCTT	3412225	1.3435522	6.909192	55-59
CTGAA	3519560	1.3298285	6.6681957	15-19
GTCAC	2365065	1.3264295	9.011389	25-29
GAGCA	2369845	1.3172427	43.278393	9
TCGTA	3361880	1.2967143	6.9242063	40-44
ACTCC	2326020	1.2894216	9.175491	20-24
TCGGA	2254630	1.2793106	43.777542	3
GCTTG	2176320	1.2606027	9.333511	55-59
TCACA	3351090	1.2515085	6.288919	30-34
CACAT	3317370	1.2389152	6.3279	30-34
AAGAG	3257300	1.2197473	29.491808	7
AGTCA	3223035	1.2177898	6.454291	25-29
GAACT	3194935	1.2071723	6.569541	20-24
AACTC	3200245	1.1951733	6.520508	20-24
TTCTG	3032770	1.194143	6.684014	55-59
TGAAC	3123685	1.1802514	6.473319	20-24
TCTGC	2021865	1.1575722	9.212938	55-59
TCTGA	2886060	1.1131852	6.54721	15-19
ATGCC	1962535	1.1006733	9.168718	45-49
CATCA	2924235	1.092094	6.2130837	30-34
GTCTT	2747160	1.0816851	6.611215	50-54
ACGAT	2852420	1.0777569	5.971056	35-39
ACATC	2844265	1.062228	6.194817	30-34
CTCGT	1823835	1.0441946	9.000423	40-44
CACGA	1899185	1.0434066	8.337431	35-39
CTTGA	2600960	1.003219	6.2481074	60-64
TCTCG	1665900	0.95377254	8.825698	40-44
CGATC	1508630	0.84610415	7.8319917	35-39
TATGC	2164060	0.83470184	6.2826705	45-49
GTATG	2116125	0.8257774	6.2749677	45-49
CGTAT	2028090	0.7822567	6.3129396	40-44
ATCTC	2043455	0.779054	5.175157	40-44
GATCT	1891280	0.7294876	5.4736686	35-39
>>END_MODULE