FastQCFastQC Report
Sun 20 Jan 2013
EL387_2KB_1.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename EL387_2KB_1.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25751908
Filtered Sequences 0
Sequence length 100
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 562799 2.185465247856586 TruSeq Adapter, Index 1 (100% over 50bp)

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGCCT 6190110 5.2135425 6.1934404 8
AGGCC 6152870 5.0764184 6.2236757 7
CACGT 8388365 4.7045536 12.692248 10-14
ACGTG 7130140 4.0457473 4.8640165 8
GCCTT 5707945 3.267952 3.671505 9
AAAAA 19269690 3.1996317 12.55412 65-69
AAGGC 5687595 3.161364 3.7519438 6
ACACG 3857810 2.1194692 9.933431 10-14
ACGTC 3736085 2.095356 9.959069 15-19
GGAAG 3599105 2.023951 44.398544 5
GAAAA 7957320 1.9842001 5.4188795 60-64
TCACG 3401530 1.9077233 10.521081 35-39
GCACA 3411350 1.8741856 9.645272 10-14
CAGTC 3241835 1.8181596 9.5395155 25-29
ATCGG 3174425 1.801216 44.334946 2
CCGTC 2100825 1.7488991 13.344373 50-54
GCCGT 2073290 1.7462022 13.576636 45-49
CGTCT 3004390 1.7200941 9.26222 15-19
TGAAA 6746140 1.7172314 5.1828566 60-64
TGCCG 1947055 1.6398823 13.767243 45-49
GTCTG 2817745 1.6321391 9.744313 15-19
GAAGA 4273985 1.6004608 29.929108 6
ATCAC 4065300 1.5182394 6.6124535 30-34
AGCAC 2725390 1.497321 9.220668 10-14
CTTCT 3772115 1.4680548 6.963211 50-54
CACAC 2671245 1.4505756 9.109162 10-14
AGAGC 2589870 1.4395403 43.458027 8
TCTTC 3573310 1.3906825 6.845307 50-54
CGGAA 2498895 1.3889732 43.030445 4
TCCAG 2472550 1.3867116 9.139956 25-29
CCAGT 2456855 1.3779092 9.152795 25-29
CTGCT 2371950 1.3580052 9.450719 55-59
CTCCA 2447090 1.3565363 9.164733 20-24
GATCG 2380180 1.3505495 43.663857 1
TGCTT 3412225 1.3435522 6.909192 55-59
CTGAA 3519560 1.3298285 6.6681957 15-19
GTCAC 2365065 1.3264295 9.011389 25-29
GAGCA 2369845 1.3172427 43.278393 9
TCGTA 3361880 1.2967143 6.9242063 40-44
ACTCC 2326020 1.2894216 9.175491 20-24
TCGGA 2254630 1.2793106 43.777542 3
GCTTG 2176320 1.2606027 9.333511 55-59
TCACA 3351090 1.2515085 6.288919 30-34
CACAT 3317370 1.2389152 6.3279 30-34
AAGAG 3257300 1.2197473 29.491808 7
AGTCA 3223035 1.2177898 6.454291 25-29
GAACT 3194935 1.2071723 6.569541 20-24
AACTC 3200245 1.1951733 6.520508 20-24
TTCTG 3032770 1.194143 6.684014 55-59
TGAAC 3123685 1.1802514 6.473319 20-24
TCTGC 2021865 1.1575722 9.212938 55-59
TCTGA 2886060 1.1131852 6.54721 15-19
ATGCC 1962535 1.1006733 9.168718 45-49
CATCA 2924235 1.092094 6.2130837 30-34
GTCTT 2747160 1.0816851 6.611215 50-54
ACGAT 2852420 1.0777569 5.971056 35-39
ACATC 2844265 1.062228 6.194817 30-34
CTCGT 1823835 1.0441946 9.000423 40-44
CACGA 1899185 1.0434066 8.337431 35-39
CTTGA 2600960 1.003219 6.2481074 60-64
TCTCG 1665900 0.95377254 8.825698 40-44
CGATC 1508630 0.84610415 7.8319917 35-39
TATGC 2164060 0.83470184 6.2826705 45-49
GTATG 2116125 0.8257774 6.2749677 45-49
CGTAT 2028090 0.7822567 6.3129396 40-44
ATCTC 2043455 0.779054 5.175157 40-44
GATCT 1891280 0.7294876 5.4736686 35-39
Produced by FastQC (version 0.10.0)