##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	600bp_2.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12354775	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.062914864900414	34.0	33.0	34.0	31.0	34.0
2	33.28192759479634	34.0	33.0	34.0	31.0	34.0
3	33.33623307587552	34.0	33.0	34.0	31.0	34.0
4	36.61337766167332	37.0	37.0	37.0	35.0	37.0
5	36.617597001968875	37.0	37.0	37.0	35.0	37.0
6	36.64592402532624	37.0	37.0	37.0	36.0	37.0
7	36.63368171415505	37.0	37.0	37.0	36.0	37.0
8	36.609824541523416	37.0	37.0	37.0	35.0	37.0
9	38.50552956245662	39.0	39.0	39.0	38.0	39.0
10-14	38.730682509394136	39.4	39.2	39.4	37.2	39.4
15-19	39.840969228496675	41.0	40.0	41.0	38.0	41.0
20-24	39.775989105426845	41.0	40.0	41.0	38.0	41.0
25-29	39.52377146487896	40.6	39.2	41.0	37.8	41.0
30-34	39.20671206072146	40.0	39.0	41.0	37.0	41.0
35-39	39.00319138146992	40.0	38.8	41.0	36.6	41.0
40-44	38.60346190035837	40.0	38.0	41.0	35.2	41.0
45-49	38.08433765892135	39.8	37.8	40.8	34.2	41.0
50-54	37.358279580162325	39.0	37.0	40.0	33.0	41.0
55-59	36.813144909559256	38.6	35.6	40.0	31.8	41.0
60-64	35.62448886361751	37.6	34.4	39.6	29.8	40.6
65-69	34.42901111513565	36.0	33.4	38.8	28.0	40.0
70-74	32.99625223445996	35.0	31.8	37.2	25.8	39.2
75-79	31.510210230457453	34.0	30.6	35.8	23.0	38.2
80-84	30.008595672523377	33.6	29.2	35.0	18.4	36.4
85-89	28.5087662867191	32.6	27.4	34.6	7.4	35.4
90-94	26.655722099350253	31.6	24.8	34.0	2.0	35.0
95-99	23.855189252738313	30.6	11.8	34.0	2.0	35.0
100-101	18.903689140433556	24.5	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14288.0
3	4320.0
4	3422.0
5	3586.0
6	3931.0
7	4267.0
8	4571.0
9	5480.0
10	6162.0
11	7210.0
12	8086.0
13	9419.0
14	10738.0
15	12299.0
16	13910.0
17	16204.0
18	19050.0
19	22400.0
20	27218.0
21	33002.0
22	39647.0
23	48895.0
24	60930.0
25	74231.0
26	93447.0
27	118653.0
28	153055.0
29	201797.0
30	271581.0
31	376008.0
32	539743.0
33	806049.0
34	1253271.0
35	2033336.0
36	3035195.0
37	2495437.0
38	515738.0
39	8191.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.326268833861906	47.084029098923594	16.921972175423882	12.667729891790616
2	17.67242614424753	34.96716937507113	31.30146201874144	16.0589424619399
3	19.181304111327684	30.242533689056966	34.96542055469575	15.610741644919607
4	19.57595675582571	30.65010094629615	32.66445366980642	17.109488628071723
5	20.085512666835946	33.578594944144314	28.34974863666279	17.98614375235695
6	19.90979196302644	32.80949268602625	25.394060191302554	21.886655159644754
7	20.192663838648873	30.39000776399024	30.54357968515368	18.87374871220721
8	17.991456744457103	30.586967387103364	30.36804798144847	21.05352788699106
9	19.82466555051297	29.86488464127401	29.79799312500263	20.51245668321039
10-14	18.924514259366685	30.562889070150568	31.339165256830615	19.173431413652132
15-19	19.440101559245885	30.160858798555545	30.525585609591015	19.87345403260755
20-24	19.65648454260252	30.316447117208412	30.525789860739334	19.50127847944973
25-29	20.00183484982036	29.886962793037082	29.930358755878572	20.180843601263984
30-34	19.92174978233564	29.789291381397966	30.36163196375594	19.927326872510452
35-39	20.12159800459073	29.842130686602196	29.678128680857906	20.358142627949167
40-44	19.974246069909572	29.843615853986353	30.16416637026535	20.017971705838722
45-49	20.186932059109758	29.85077956569367	29.799350649014244	20.162937726182324
50-54	19.98372592229433	29.87162604207294	30.15472175545957	19.989926280173158
55-59	20.083605754039453	29.955533500619957	29.802013406467527	20.158847338873066
60-64	20.048619837493327	30.091221364987547	29.945953711270125	19.914205086249005
65-69	20.24638962237924	29.8788022100822	29.698468081032374	20.176340086506183
70-74	19.843347831310794	30.19741063159041	29.775684055962092	20.183557481136706
75-79	19.84876757907444	30.143152116017607	30.087351633161312	19.920728671746645
80-84	19.834450274125523	30.000838711286843	30.1564818986727	20.008229115914936
85-89	20.137604555404994	29.634845190990028	29.959591138816766	20.26795911478821
90-94	19.98573360502208	29.2307518968722	30.24836086118272	20.535153636923003
95-99	20.163233658628496	29.303138077613383	30.115162119000605	20.418466144757517
100-101	21.55549160357794	28.756673593688394	28.537118477405848	21.15071632532782
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	35.993998725652524
2	33.73136860618743
3	34.79204575624728
4	36.68544538389743
5	38.0716564191929
6	41.796447122671196
7	39.06641255085608
8	39.044984631448166
9	40.33712223372336
10-14	38.09794567301882
15-19	39.31355559185344
20-24	39.15776302205225
25-29	40.18267845108434
30-34	39.849076654846094
35-39	40.479740632539894
40-44	39.99221777574829
45-49	40.349869785292086
50-54	39.97365220246749
55-59	40.24245309291252
60-64	39.96282492374233
65-69	40.42272970888543
70-74	40.0269053124475
75-79	39.769496250821085
80-84	39.84267939004046
85-89	40.4055636701932
90-94	40.52088724194508
95-99	40.58169980338601
100-101	42.70620792890576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	514.0
1	305.0
2	79.0
3	61.5
4	69.0
5	71.5
6	86.0
7	102.5
8	124.0
9	206.0
10	317.0
11	448.0
12	754.5
13	1217.5
14	1851.0
15	2692.5
16	4046.0
17	6271.0
18	9357.5
19	14011.0
20	21043.5
21	30431.5
22	42501.0
23	58172.5
24	78039.0
25	106288.5
26	134059.0
27	161155.5
28	197897.5
29	242604.0
30	294548.0
31	352930.0
32	421849.5
33	502621.5
34	579636.0
35	628356.0
36	641184.5
37	621968.0
38	577761.0
39	520847.0
40	462545.0
41	408983.0
42	362732.5
43	328538.0
44	315588.0
45	331696.5
46	375611.5
47	436097.0
48	491337.0
49	516950.0
50	497797.0
51	434233.5
52	344891.0
53	256312.5
54	182115.0
55	124564.5
56	83510.0
57	54605.0
58	34597.5
59	21254.0
60	12297.5
61	7133.5
62	4282.0
63	2719.0
64	1878.5
65	1385.0
66	1032.5
67	801.5
68	645.5
69	523.5
70	390.5
71	304.5
72	262.5
73	202.5
74	161.0
75	131.0
76	106.0
77	76.0
78	54.5
79	40.0
80	33.0
81	27.0
82	18.0
83	15.5
84	14.0
85	13.0
86	13.5
87	9.0
88	4.0
89	4.0
90	5.0
91	4.0
92	2.0
93	1.5
94	1.5
95	1.5
96	1.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06527031046700567
2	0.0061595617888630105
3	0.0049778324574911315
4	0.0015054907920217082
5	0.0028167247076535187
6	0.0
7	0.00245249306442246
8	0.0
9	0.003585658176696864
10-14	0.003103253600328618
15-19	0.0017240297779603433
20-24	0.003962840278353915
25-29	0.005102480619841316
30-34	0.00536148978836118
35-39	0.00532425722038645
40-44	0.0037750586311770147
45-49	0.008293149814545387
50-54	0.0052562673136499856
55-59	0.001382461436974773
60-64	0.003854380189036223
65-69	0.0030336448862889045
70-74	3.253802679530789E-4
75-79	3.496623775018161E-4
80-84	8.612054853285471E-4
85-89	7.770275055595914E-5
90-94	0.0011914421751913734
95-99	0.0025350522368881665
100-101	1.4973967555054625E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2354775E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	36.186956541358136
#Duplication Level	Relative count
1	100.0
2	10.467550099704422
3	4.050403767554342
4	2.5831223748037733
5	1.943882674058465
6	1.496273458824195
7	1.3986904071617474
8	1.1405903067502015
9	1.0316932780834123
10++	17.312506187331174
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGCCT	3130615	5.548993	9.20329	3
AGGCC	3091550	5.441085	8.600624	2
CACGT	3746870	4.3825135	6.8143945	4
ACGTG	3669830	4.2822685	7.156758	5
GCCTT	2849365	3.3564332	5.8887825	4
AAGGC	2777440	3.2180836	6.1147933	1
GGCGC	1173855	3.1307874	4.315023	50-54
GCGCC	1149745	3.0737417	3.9401164	15-19
GCCTG	1699760	3.0128129	4.0566707	5
CGCGC	1126320	3.0111175	4.2457604	45-49
>>END_MODULE