##FastQC	0.10.0
>>Basic Statistics	pass
#Measure	Value	
Filename	600bp_1.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	12354775	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.48731134318513	34.0	34.0	34.0	31.0	34.0
2	33.642502837971556	34.0	34.0	34.0	33.0	34.0
3	33.68409542059649	34.0	34.0	34.0	33.0	34.0
4	36.81671863712613	37.0	37.0	37.0	37.0	37.0
5	36.722097812384284	37.0	37.0	37.0	37.0	37.0
6	36.77612777246045	37.0	37.0	37.0	37.0	37.0
7	36.788485666473086	37.0	37.0	37.0	37.0	37.0
8	36.786848242885846	37.0	37.0	37.0	37.0	37.0
9	38.72585692576352	39.0	39.0	39.0	38.0	39.0
10-14	38.98909437039525	39.4	39.2	39.4	38.2	39.4
15-19	40.09779409175805	41.0	40.0	41.0	38.2	41.0
20-24	39.9538506690733	41.0	40.0	41.0	38.0	41.0
25-29	39.785015251188305	41.0	40.0	41.0	38.0	41.0
30-34	39.488684787865424	40.2	39.4	41.0	37.6	41.0
35-39	39.03308366198494	40.0	38.6	41.0	36.6	41.0
40-44	38.39770028996885	40.0	38.0	41.0	34.8	41.0
45-49	37.67904655487453	39.6	37.6	40.2	33.4	41.0
50-54	36.62370939171292	38.8	36.0	40.0	31.0	41.0
55-59	35.48551181223454	38.0	34.4	39.8	28.8	40.6
60-64	33.96720555412786	36.6	32.8	39.0	26.0	40.0
65-69	32.079002604256246	35.0	30.8	37.8	21.2	39.4
70-74	29.89254674407264	33.6	28.4	36.2	10.4	38.6
75-79	27.835031767069818	32.2	26.0	35.0	2.0	37.0
80-84	26.049896044242008	31.2	23.0	34.0	2.0	35.6
85-89	23.6660207247805	29.6	13.2	34.0	2.0	35.0
90-94	21.02684152483554	27.6	2.0	32.6	2.0	34.2
95-99	17.62494188684132	20.8	2.0	31.6	2.0	34.0
100-101	14.180973388831443	2.0	2.0	30.0	2.0	33.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75.0
3	67.0
4	120.0
5	299.0
6	766.0
7	1833.0
8	3441.0
9	5590.0
10	8137.0
11	11182.0
12	13759.0
13	16905.0
14	21244.0
15	26094.0
16	31809.0
17	38946.0
18	48113.0
19	60004.0
20	76589.0
21	94636.0
22	117341.0
23	145591.0
24	178445.0
25	212530.0
26	248337.0
27	287431.0
28	339390.0
29	409153.0
30	494256.0
31	606363.0
32	766768.0
33	1006645.0
34	1383039.0
35	1929804.0
36	2303300.0
37	1319744.0
38	146101.0
39	928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.785348393621838	46.76224437637123	16.874403296977704	12.57800393302923
2	17.852529891232145	34.45975109704338	31.738845348477753	15.94887366324673
3	19.115772829316626	30.036032303349753	35.27130116776915	15.57689369956447
4	19.446343620179242	30.904480251562656	32.860153260581434	16.789022867676668
5	20.152509178875672	33.75166278403607	28.34752050682605	17.748307530262203
6	19.949412271773465	33.068477572436564	25.50844511535014	21.47366504043983
7	20.202537075746015	30.750976849032053	30.37056522680502	18.675920848416908
8	18.13744078706411	30.777282467709853	30.14946852532725	20.93580821989878
9	19.99090230295574	30.053343747660318	29.588049964487418	20.36770398489653
10-14	19.05514588489066	30.777295418168276	31.13181259877254	19.03574609816852
15-19	19.59272828759455	30.40243299123947	30.283828472595903	19.721010248570074
20-24	19.781989849713195	30.544060080078257	30.30366374778727	19.370286322421272
25-29	20.124841827388472	30.092381029038744	29.726638938932048	20.05613820464073
30-34	20.064464961010195	29.99164547547919	30.158326406573615	19.785563156937
35-39	20.271978634308944	30.08155567594324	29.44299514424354	20.203470545504278
40-44	20.12511317190821	30.025349276781473	29.97158735770199	19.877950193608328
45-49	20.363476101518767	30.064569445298815	29.556048033308617	20.015906419873797
50-54	20.238965735959873	29.980082438417977	29.896316916866756	19.884634908755398
55-59	20.347853924817812	30.074692919064912	29.514192094938018	20.063261061179258
60-64	20.317748049016664	30.16331716678363	29.740769431414254	19.77816535278545
65-69	20.598318050518962	29.921029344781058	29.393025282695817	20.08762732200416
70-74	20.280274014722853	30.190556148912478	29.424292297825282	20.10487753853938
75-79	20.215306303228502	30.173483053259776	29.660952525505518	19.9502581180062
80-84	20.180317956762348	29.98336963586577	29.72473372072223	20.111578686649654
85-89	20.524892260828963	29.589601426626395	29.377972054004854	20.507534258539785
90-94	20.379383313522727	29.15810619934159	29.609351301958895	20.853159185176796
95-99	20.571119729706187	29.290061410794994	29.27155838101695	20.867260478481874
100-101	21.29614663156553	28.922768727071112	28.28715213348685	21.493932507876508
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	36.363352326651075
2	33.80140355447887
3	34.69266652888109
4	36.235366487855906
5	37.90081670913787
6	41.423077312213294
7	38.87845792416292
8	39.0732490069629
9	40.35860628785227
10-14	38.09089198305918
15-19	39.31373853616462
20-24	39.15227617213447
25-29	40.180980032029204
30-34	39.8500281179472
35-39	40.475449179813225
40-44	40.00306336551654
45-49	40.379382521392564
50-54	40.12360064471527
55-59	40.411114985997074
60-64	40.095913401802115
65-69	40.68594537252312
70-74	40.38515155326223
75-79	40.1655644212347
80-84	40.291896643412
85-89	41.032426519368755
90-94	41.23254249869952
95-99	41.438380208188065
100-101	42.79007913944204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	485.0
1	302.5
2	95.5
3	66.0
4	56.0
5	64.0
6	76.5
7	80.5
8	103.0
9	164.5
10	271.0
11	448.5
12	745.5
13	1160.5
14	1707.5
15	2537.0
16	3929.5
17	6165.0
18	9229.5
19	13783.0
20	20766.5
21	29934.0
22	42284.0
23	57919.0
24	77646.5
25	105824.0
26	133990.5
27	161852.5
28	197919.5
29	242427.0
30	294832.5
31	353441.0
32	422440.0
33	501362.5
34	575638.0
35	622547.0
36	634545.0
37	615332.5
38	571545.5
39	515294.0
40	457457.0
41	403314.0
42	357345.5
43	324904.5
44	312828.5
45	327505.0
46	366333.5
47	418090.5
48	463649.5
49	483511.0
50	467083.0
51	415073.0
52	341215.0
53	266330.0
54	201884.5
55	150096.5
56	109743.5
57	79451.0
58	56909.0
59	39891.5
60	27567.0
61	19116.5
62	13376.5
63	9166.5
64	6346.5
65	4511.0
66	3273.0
67	2390.0
68	1714.5
69	1267.5
70	967.5
71	773.5
72	599.0
73	463.5
74	375.5
75	324.5
76	257.0
77	191.5
78	151.5
79	114.5
80	90.5
81	72.0
82	59.0
83	50.5
84	39.0
85	33.0
86	28.0
87	19.5
88	14.5
89	11.0
90	7.5
91	4.5
92	3.0
93	4.0
94	2.5
95	0.0
96	1.5
97	1.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0034804357019856693
2	0.08650096824911825
3	0.01819539408852043
4	0.0
5	0.07093613602837769
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0021934838959025964
20-24	8.741559437545402E-5
25-29	0.004453338891238408
30-34	2.6224678312636204E-4
35-39	0.004964881999065139
40-44	0.0295707530084522
45-49	1.2141054774368615E-4
50-54	4.0308301850903804E-4
55-59	2.266330224548808E-5
60-64	4.208898988447786E-5
65-69	0.0010943137369964246
70-74	1.8454403257040295E-4
75-79	9.372894285812571E-4
80-84	0.002144919676805122
85-89	0.006627397099502014
90-94	9.275765847617622E-4
95-99	3.367119190758229E-4
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.2354775E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.55256577516852
#Duplication Level	Relative count
1	100.0
2	10.177199530466932
3	4.083813058361273
4	2.5824318720170942
5	1.880943845813213
6	1.5548011161953763
7	1.2989477679606942
8	1.1239272083166396
9	1.0009207908961193
10++	16.87577757628155
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGCCT	3022755	5.3064914	8.288268	3
AGGCC	3067805	5.2737517	7.8425975	2
CACGT	3602640	4.2077465	6.785112	4
ACGTG	3490440	4.012658	7.152729	5
GCCTT	2725545	3.2508326	5.5056734	7
GGCGC	1227585	3.171899	4.209604	50-54
AAGGC	2766470	3.114336	5.6375046	1
CGCGC	1154790	3.0314298	4.4239883	90-94
GCCTG	1717760	3.015553	3.941014	5
GCGCC	1148580	3.015128	3.810545	15-19
GCGCG	1164435	3.0087287	4.256458	40-44
GGGGG	972795	2.435206	8.276648	95-97
>>END_MODULE